ELISA Kit for Advanced Glycation End Product (AGE)
AGEs; Advanced Glycation End Products
- Product No.CEB353Ge
- Organism SpeciesPan-species (General)Same name, Different species.
- Sample Typeserum, plasma and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range98.8-8000ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 38.2ng/mL.
- DownloadInstruction Manual
- FOB US$ 497
For negotiated price and more details, please contact local distributors! US$ 711
For negotiated price and more details, please contact local distributors! US$ 3198
For negotiated price and more details, please contact local distributors! US$ 6040
For negotiated price and more details, please contact local distributors! US$ 49742
For negotiated price and more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Advanced Glycation End Product (AGE).
No significant cross-reactivity or interference between Advanced Glycation End Product (AGE) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Advanced Glycation End Product (AGE) and the recovery rates were calculated by comparing the measured value to the expected amount of Advanced Glycation End Product (AGE) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Advanced Glycation End Product (AGE) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Advanced Glycation End Product (AGE) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Advanced Glycation End Product (AGE) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Advanced Glycation End Product (AGE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Advanced Glycation End Product (AGE) and unlabeled Advanced Glycation End Product (AGE) (Standards or samples) with the pre-coated antibody specific to Advanced Glycation End Product (AGE). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Advanced Glycation End Product (AGE) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Advanced Glycation End Product (AGE) in the sample.
|Human Immunology||Threshold serum concentrations of tumour necrosis factor alpha (TNFα) as a potential marker of the presence of microangiopathy in children and adolescents with type 1 diabetes mellitus (T1DM)PubMed: S0198885912005642|
|Experimental Dermatology||A long-standing hyperglycaemic condition impairs skin barrier by accelerating skin ageing processPubMed: 22017743|
|J Hum Nutr Diet.||Daily intake of vitamin D- or calcium-vitamin D-fortified Persian yogurt drink (doogh) attenuates diabetes-induced oxidative stress: evidence for antioxidative properties of vitamin D.Pubmed: 23829785|
|Gene||Association of RAGE gene polymorphism with circulating AGEs level and paraoxonase activity in relation to macro-vascular complications in Indian type 2 diabetes mellitus patients.Pubmed: 23721855|
|Gynecol Endocrinol.||Lipoprotein-associated phospholipase A2 and AGEs are associated with cardiovascular risk factors in women with history of gestational diabetes mellitus.Pubmed:24397392|
|Microvasc Res.||Role of advanced glycation end product (AGE)-induced receptor (RAGE) expression in diabetic vascular complicationsPubmed:24984291|
|Free Radic Res.||Nitroxides prevent protein glycoxidation in vitro.Pubmed:25363554|
|Sleep and Breathing||Serum advanced glycation end products are associated with insulin resistance in male nondiabetic patients with obstructive sleep apneaPubmed:25566941|
|Chem Biol Interact||Ascorbic acid and protein glycationPubMed: 26163454|
|Osteoarthritis Cartilage||Establishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of pioglitazonePubMed: 26321377|
|journal of human hypertension||Relationship between serum levels of endogenous secretory RAGE and blood pressure in male nondiabetic patients with obstructive sleep apneaPubMed: 25994997|
|Redox Biol.||Glycation of bovine serum albumin by ascorbatePubMed: 26202868|
|J Oral Pathol Med||Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human salivaPubMed: 26608886|
|J Agric Food Chem||Inhibition of Advanced Glycation Endproduct Formation by Lotus Seedpod Oligomeric Procyanidins through RAGE-MAPK Signaling and NF-κB Activation in High-Fat-Diet Rats.PubMed: 26207852|
|Kineziologija||ADVANCED GLYCATION END PRODUCTS'RESPONSE TO RESISTANCE TRAINING IN POSTMENOPAUSAL WOMEN WITH TYPE II DIABETES140251:|
|Food Funct.||Effects of edible bird's nest on hippocampal and cortical neurodegeneration in ovariectomized ratsPubMed: 25920003|
|Molecular and Cellular Endocrinology||Beneficial effects of urine-derived stem cells on fibrosis and apoptosis of myocardial, glomerular and bladder cellsPubmed:26952874|
|Advances in Experimental Medicine and Biology||Effects of S-Nitroso-N-Acetyl-Penicillamine (SNAP) on Inflammation, Lung Tissue Apoptosis and iNOS Activity in a Rabbit Model of Acute Lung InjuryPubmed:27334732|
|Eur J Vasc Endovasc Surg||The Receptor for Advanced Glycation End Products (Rage) and Its Ligands in Plasma and InfrainCavia (Guinea pig )inal Bypass VeinPubmed:26905625|
|Catalog No.||Related products for research use of Pan-species (General) Organism species||Applications|
|NPB353Ge01||Native Advanced Glycation End Product (AGE)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APB353Ge01||Active Advanced Glycation End Product (AGE)||Cell culture; Activity Assays.|
|PAB353Ge01||Polyclonal Antibody to Advanced Glycation End Product (AGE)||WB; IHC; ICC; IP.|
|LAB353Ge71||Biotin-Linked Polyclonal Antibody to Advanced Glycation End Product (AGE)||WB; IHC; ICC.|
|LAB353Ge81||FITC-Linked Polyclonal Antibody to Advanced Glycation End Product (AGE)||WB; IHC; ICC; IF.|
|MAB353Ge21||Monoclonal Antibody to Advanced Glycation End Product (AGE)||WB; IHC; ICC; IP.|
|CEB353Ge||ELISA Kit for Advanced Glycation End Product (AGE)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSB353Ge11||ELISA Kit DIY Materials for Advanced Glycation End Product (AGE)||Main materials for "Do It (ELISA Kit) Yourself".|