ELISA Kit for Advanced Glycation End Product (AGE)

AGEs; Advanced Glycation End Products

Specificity

This assay has high sensitivity and excellent specificity for detection of Advanced Glycation End Product (AGE).
No significant cross-reactivity or interference between Advanced Glycation End Product (AGE) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Advanced Glycation End Product (AGE) and the recovery rates were calculated by comparing the measured value to the expected amount of Advanced Glycation End Product (AGE) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)84-9389
EDTA plasma(n=5)85-9591
heparin plasma(n=5)87-10398

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Advanced Glycation End Product (AGE) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Advanced Glycation End Product (AGE) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Advanced Glycation End Product (AGE) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)80-101%79-98%98-105%90-102%
EDTA plasma(n=5)99-105%80-101%81-90%95-104%
heparin plasma(n=5)82-98%89-101%87-103%80-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×120µLAssay Diluent A1×12mL
Detection Reagent B1×120µLAssay Diluent B1×12mL
TMB Substrate1×9mLStop Solution1×6mL
Wash Buffer (30 × concentrate)1×20mLInstruction manual1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

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MagazineReference
Human ImmunologyThreshold serum concentrations of tumour necrosis factor alpha (TNFα) as a potential marker of the presence of microangiopathy in children and adolescents with type 1 diabetes mellitus (T1DM)PubMed: S0198885912005642
Experimental DermatologyA long-standing hyperglycaemic condition impairs skin barrier by accelerating skin ageing processPubMed: 22017743
J Hum Nutr Diet. Daily intake of vitamin D- or calcium-vitamin D-fortified Persian yogurt drink (doogh) attenuates diabetes-induced oxidative stress: evidence for antioxidative properties of vitamin D.Pubmed: 23829785
GeneAssociation of RAGE gene polymorphism with circulating AGEs level and paraoxonase activity in relation to macro-vascular complications in Indian type 2 diabetes mellitus patients.Pubmed: 23721855
Gynecol Endocrinol. Lipoprotein-associated phospholipase A2 and AGEs are associated with cardiovascular risk factors in women with history of gestational diabetes mellitus.Pubmed:24397392
Microvasc Res.Role of advanced glycation end product (AGE)-induced receptor (RAGE) expression in diabetic vascular complicationsPubmed:24984291
Free Radic Res.Nitroxides prevent protein glycoxidation in vitro.Pubmed:25363554
Sleep and BreathingSerum advanced glycation end products are associated with insulin resistance in male nondiabetic patients with obstructive sleep apneaPubmed:25566941
Chem Biol InteractAscorbic acid and protein glycationPubMed: 26163454
Osteoarthritis CartilageEstablishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of pioglitazonePubMed: 26321377
journal of human hypertensionRelationship between serum levels of endogenous secretory RAGE and blood pressure in male nondiabetic patients with obstructive sleep apneaPubMed: 25994997
Redox Biol.Glycation of bovine serum albumin by ascorbatePubMed: 26202868
J Oral Pathol MedImpact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human salivaPubMed: 26608886
J Agric Food ChemInhibition of Advanced Glycation Endproduct Formation by Lotus Seedpod Oligomeric Procyanidins through RAGE-MAPK Signaling and NF-κB Activation in High-Fat-Diet Rats.PubMed: 26207852
KineziologijaADVANCED GLYCATION END PRODUCTS&#039;RESPONSE TO RESISTANCE TRAINING IN POSTMENOPAUSAL WOMEN WITH TYPE II DIABETES140251:
Food Funct.Effects of edible bird&#039;s nest on hippocampal and cortical neurodegeneration in ovariectomized ratsPubMed: 25920003
Molecular and Cellular EndocrinologyBeneficial effects of urine-derived stem cells on fibrosis and apoptosis of myocardial, glomerular and bladder cellsPubmed:26952874
Advances in Experimental Medicine and BiologyEffects of S-Nitroso-N-Acetyl-Penicillamine (SNAP) on Inflammation, Lung Tissue Apoptosis and iNOS Activity in a Rabbit Model of Acute Lung InjuryPubmed:27334732
Eur J Vasc Endovasc SurgThe Receptor for Advanced Glycation End Products (Rage) and Its Ligands in Plasma and InfrainCavia (Guinea pig )inal Bypass VeinPubmed:26905625
molecular and cellular endocrinologyBerberine exerts renoprotective effects by regulating the AGEs-RAGE signaling pathway in mesangial cells during diabetic nephropathy.pubmed:28087385
cellular physiology and biochemistryInhibition of Methylglyoxal-Induced AGEs/RAGE Expression Contributes to Dermal Protection by N-Acetyl-L-Cysteine.pubmed:28214842
General Physiology and BiophysicsGlutathione is the main endogenous inhibitor of protein glycation.pubmed:28218612
Osteoarthritis and CartilageEstablishment of a rabbit model to study the influence of advanced glycation end productsaccumulation on osteoarthritis and the protective effect of pioglitazone.pubmed:26321377
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