ELISA Kit DIY Materials for Advanced Glycation End Product (AGE)
AGEs; Advanced Glycation End Products
- Product No.KSB353Ge11
- Organism SpeciesPan-species (General) Same name, Different species.
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range98.77-8,000ng/m
- Applicable Sensitivity38.2ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- DownloadInstruction Manual
- UOM 96T*596T*10 96T*20 96T*50 96T*100
For more details, please contact local distributors! US$ 2133
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The Abs in the kit have high sensitivity and excellent specificity for detection of Advanced Glycation End Product (AGE). No significant cross-reactivity or interference between Advanced Glycation End Product (AGE) and analogues was observed.
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
|Human Immunology||Threshold serum concentrations of tumour necrosis factor alpha (TNFα) as a potential marker of the presence of microangiopathy in children and adolescents with type 1 diabetes mellitus (T1DM) PubMed: S0198885912005642|
|Experimental Dermatology||A long-standing hyperglycaemic condition impairs skin barrier by accelerating skin ageing process PubMed: 22017743|
|J Hum Nutr Diet.||Daily intake of vitamin D- or calcium-vitamin D-fortified Persian yogurt drink (doogh) attenuates diabetes-induced oxidative stress: evidence for antioxidative properties of vitamin D. Pubmed: 23829785|
|Gene||Association of RAGE gene polymorphism with circulating AGEs level and paraoxonase activity in relation to macro-vascular complications in Indian type 2 diabetes mellitus patients. Pubmed: 23721855|
|Gynecol Endocrinol.||Lipoprotein-associated phospholipase A2 and AGEs are associated with cardiovascular risk factors in women with history of gestational diabetes mellitus. Pubmed:24397392|
|Microvasc Res.||Role of advanced glycation end product (AGE)-induced receptor (RAGE) expression in diabetic vascular complications Pubmed:24984291|
|Free Radic Res.||Nitroxides prevent protein glycoxidation in vitro. Pubmed:25363554|
|Sleep and Breathing||Serum advanced glycation end products are associated with insulin resistance in male nondiabetic patients with obstructive sleep apnea Pubmed:25566941|
|Chem Biol Interact||Ascorbic acid and protein glycation PubMed: 26163454|
|Osteoarthritis Cartilage||Establishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of pioglitazone PubMed: 26321377|
|journal of human hypertension||Relationship between serum levels of endogenous secretory RAGE and blood pressure in male nondiabetic patients with obstructive sleep apnea PubMed: 25994997|
|Redox Biol.||Glycation of bovine serum albumin by ascorbate PubMed: 26202868|
|J Oral Pathol Med||Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human saliva PubMed: 26608886|
|J Agric Food Chem||Inhibition of Advanced Glycation Endproduct Formation by Lotus Seedpod Oligomeric Procyanidins through RAGE-MAPK Signaling and NF-κB Activation in High-Fat-Diet Rats. PubMed: 26207852|
|Kineziologija||ADVANCED GLYCATION END PRODUCTS'RESPONSE TO RESISTANCE TRAINING IN POSTMENOPAUSAL WOMEN WITH TYPE II DIABETES 140251:|
|Food Funct.||Effects of edible bird's nest on hippocampal and cortical neurodegeneration in ovariectomized rats PubMed: 25920003|
|Molecular and Cellular Endocrinology||Beneficial effects of urine-derived stem cells on fibrosis and apoptosis of myocardial, glomerular and bladder cells Pubmed:26952874|
|Advances in Experimental Medicine and Biology||Effects of S-Nitroso-N-Acetyl-Penicillamine (SNAP) on Inflammation, Lung Tissue Apoptosis and iNOS Activity in a Rabbit Model of Acute Lung Injury Pubmed:27334732|
|Eur J Vasc Endovasc Surg||The Receptor for Advanced Glycation End Products (Rage) and Its Ligands in Plasma and InfrainCavia (Guinea pig )inal Bypass Vein Pubmed:26905625|
|molecular and cellular endocrinology||Berberine exerts renoprotective effects by regulating the AGEs-RAGE signaling pathway in mesangial cells during diabetic nephropathy. pubmed:28087385|
|cellular physiology and biochemistry||Inhibition of Methylglyoxal-Induced AGEs/RAGE Expression Contributes to Dermal Protection by N-Acetyl-L-Cysteine. pubmed:28214842|
|General Physiology and Biophysics||Glutathione is the main endogenous inhibitor of protein glycation. pubmed:28218612|
|Osteoarthritis and Cartilage||Establishment of a rabbit model to study the influence of advanced glycation end productsaccumulation on osteoarthritis and the protective effect of pioglitazone. pubmed:26321377|
|Scientific Reports||Advanced glycation end products promote VEGF expression and thus choroidal neovascularization via Cyr61-PI3K/AKT signaling pathway pubmed:29097668|
|INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE||Lychee seed extract protects against neuronal injury and improves cognitive function in rats with type II diabetes mellitus with cognitive impairment pubmed:29138799|
|Catalog No.||Related products for research use of Pan-species (General) Organism species||Applications (RESEARCH USE ONLY!)|
|NPB353Ge01||Native Advanced Glycation End Product (AGE)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APB353Ge01||Active Advanced Glycation End Product (AGE)||Cell culture; Activity Assays.|
|PAB353Ge01||Polyclonal Antibody to Advanced Glycation End Product (AGE)||WB; IHC; ICC; IP.|
|LAB353Ge71||Biotin-Linked Polyclonal Antibody to Advanced Glycation End Product (AGE)||WB; IHC; ICC.|
|LAB353Ge81||FITC-Linked Polyclonal Antibody to Advanced Glycation End Product (AGE)||WB; IHC; ICC; IF.|
|MAB353Ge21||Monoclonal Antibody to Advanced Glycation End Product (AGE)||WB; IHC; ICC; IP.|
|HEB353Ge||High Sensitive ELISA Kit for Advanced Glycation End Product (AGE)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|CEB353Ge||ELISA Kit for Advanced Glycation End Product (AGE)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSB353Ge11||ELISA Kit DIY Materials for Advanced Glycation End Product (AGE)||Main materials for "Do It (ELISA Kit) Yourself".|