ELISA Kit DIY Materials for Advanced Glycation End Product (AGE)

AGEs; Advanced Glycation End Products


The Abs in the kit have high sensitivity and excellent specificity for detection of Advanced Glycation End Product (AGE). No significant cross-reactivity or interference between Advanced Glycation End Product (AGE) and analogues was observed.


1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.


Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Human Immunology Threshold serum concentrations of tumour necrosis factor alpha (TNFα) as a potential marker of the presence of microangiopathy in children and adolescents with type 1 diabetes mellitus (T1DM) PubMed: S0198885912005642
Experimental Dermatology A long-standing hyperglycaemic condition impairs skin barrier by accelerating skin ageing process PubMed: 22017743
J Hum Nutr Diet. Daily intake of vitamin D- or calcium-vitamin D-fortified Persian yogurt drink (doogh) attenuates diabetes-induced oxidative stress: evidence for antioxidative properties of vitamin D. Pubmed: 23829785
Gene Association of RAGE gene polymorphism with circulating AGEs level and paraoxonase activity in relation to macro-vascular complications in Indian type 2 diabetes mellitus patients. Pubmed: 23721855
Gynecol Endocrinol. Lipoprotein-associated phospholipase A2 and AGEs are associated with cardiovascular risk factors in women with history of gestational diabetes mellitus. Pubmed:24397392
Microvasc Res. Role of advanced glycation end product (AGE)-induced receptor (RAGE) expression in diabetic vascular complications Pubmed:24984291
Free Radic Res. Nitroxides prevent protein glycoxidation in vitro. Pubmed:25363554
Sleep and Breathing Serum advanced glycation end products are associated with insulin resistance in male nondiabetic patients with obstructive sleep apnea Pubmed:25566941
Chem Biol Interact Ascorbic acid and protein glycation PubMed: 26163454
Osteoarthritis Cartilage Establishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of pioglitazone PubMed: 26321377
journal of human hypertension Relationship between serum levels of endogenous secretory RAGE and blood pressure in male nondiabetic patients with obstructive sleep apnea PubMed: 25994997
Redox Biol. Glycation of bovine serum albumin by ascorbate PubMed: 26202868
J Oral Pathol Med Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human saliva PubMed: 26608886
J Agric Food Chem Inhibition of Advanced Glycation Endproduct Formation by Lotus Seedpod Oligomeric Procyanidins through RAGE-MAPK Signaling and NF-κB Activation in High-Fat-Diet Rats. PubMed: 26207852
Food Funct. Effects of edible bird's nest on hippocampal and cortical neurodegeneration in ovariectomized rats PubMed: 25920003
Molecular and Cellular Endocrinology Beneficial effects of urine-derived stem cells on fibrosis and apoptosis of myocardial, glomerular and bladder cells Pubmed:26952874
Advances in Experimental Medicine and Biology Effects of S-Nitroso-N-Acetyl-Penicillamine (SNAP) on Inflammation, Lung Tissue Apoptosis and iNOS Activity in a Rabbit Model of Acute Lung Injury Pubmed:27334732
Eur J Vasc Endovasc Surg The Receptor for Advanced Glycation End Products (Rage) and Its Ligands in Plasma and InfrainCavia (Guinea pig )inal Bypass Vein Pubmed:26905625
molecular and cellular endocrinology Berberine exerts renoprotective effects by regulating the AGEs-RAGE signaling pathway in mesangial cells during diabetic nephropathy. pubmed:28087385
cellular physiology and biochemistry Inhibition of Methylglyoxal-Induced AGEs/RAGE Expression Contributes to Dermal Protection by N-Acetyl-L-Cysteine. pubmed:28214842
General Physiology and Biophysics Glutathione is the main endogenous inhibitor of protein glycation. pubmed:28218612
Osteoarthritis and Cartilage Establishment of a rabbit model to study the influence of advanced glycation end productsaccumulation on osteoarthritis and the protective effect of pioglitazone. pubmed:26321377
Scientific Reports Advanced glycation end products promote VEGF expression and thus choroidal neovascularization via Cyr61-PI3K/AKT signaling pathway pubmed:29097668
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Lychee seed extract protects against neuronal injury and improves cognitive function in rats with type II diabetes mellitus with cognitive impairment pubmed:29138799
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
NPB353Ge01 Native Advanced Glycation End Product (AGE) Positive Control; Immunogen; SDS-PAGE; WB.
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LAB353Ge71 Biotin-Linked Polyclonal Antibody to Advanced Glycation End Product (AGE) WB; IHC; ICC.
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KSB353Ge11 ELISA Kit DIY Materials for Advanced Glycation End Product (AGE) Main materials for "Do It (ELISA Kit) Yourself".