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Human Hacat cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human P53 Monoclonal Antibody (Catalog FAA928Hu04, red histogram) or Isotype control antibody (Catalog IS067-Mu01, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
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Anti-Tumor Protein p53 (P53) Monoclonal Antibody
TP53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
- Product No.FAA928Hu04
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Potency (Clone Number)D2
- Ig Isotype IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Excitation Laser
- Vol. per test
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Organism Species Moren/a
- ApplicationsFlow cytometry.
- Downloadn/a
- UOM 25test50test 100test 200test 500test
- FOB
US$
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For more details, please contact local distributors!
USAGE
Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
GIVEAWAYS
INCREMENT SERVICES
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Protein A/G Purification Column
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Isotype Control
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Real Time PCR Experimental Service
| Magazine | Citations |
| Anticancer Research | Analysis of p53 and miRNA expression after irradiation of glioblastoma cell lines PubMed: 23155233 |
| Microscopy Research and Technique | Therapeutic role of curcumin in oxidative DNA damage caused by formaldehyde PubMed: 25761397 |
| J Nutr Biochem | Supplementation with Selenium yeast on the prooxidant–antioxidant activities and anti-tumor effects in breast tumor xenograft-bearing mice PubMed: 26344777 |
| Biochemical and Biophysical Research Communications | Transcription factor HBP1: A regulator of senescence and apoptosis of preadipocytes Pubmed: 31331641 |
| Folia Biologica | Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β … |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA928Hu01 | Recombinant Tumor Protein p53 (P53) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA928Hu02 | Recombinant Tumor Protein p53 (P53) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA928Hu03 | Recombinant Tumor Protein p53 (P53) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA928Hu01 | Active Tumor Protein p53 (P53) | Cell culture; Activity Assays. |
| PAA928Hu01 | Polyclonal Antibody to Tumor Protein p53 (P53) | WB,ICC/IF |
| PAA928Hu02 | Polyclonal Antibody to Tumor Protein p53 (P53) | WB; IHC; ICC; IP. |
| LAA928Hu71 | Biotin-Linked Polyclonal Antibody to Tumor Protein p53 (P53) | WB; IHC; ICC. |
| MAA928Hu22 | Monoclonal Antibody to Tumor Protein p53 (P53) | WB |
| MAA928Hu24 | Monoclonal Antibody to Tumor Protein p53 (P53) | WB; IHC; ICC; IP. |
| MAA928Hu25 | Monoclonal Antibody to Tumor Protein p53 (P53) | WB; IHC; ICC; IP. |
| MAA928Hu27 | Monoclonal Antibody to Tumor Protein p53 (P53) | WB; IHC; ICC; IP. |
| MAA928Hu21 | Monoclonal Antibody to Tumor Protein p53 (P53) | WB; IHC; ICC; IP. |
| MAA928Hu23 | Monoclonal Antibody to Tumor Protein p53 (P53) | WB; IHC; ICC; IP. |
| FAA928Hu04 | Anti-Tumor Protein p53 (P53) Monoclonal Antibody | Flow cytometry. |
| SEA928Hu | ELISA Kit for Tumor Protein p53 (P53) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA928Hu | CLIA Kit for Tumor Protein p53 (P53) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA928Hu | Multiplex Assay Kit for Tumor Protein p53 (P53) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSA928Hu01 | ELISA Kit DIY Materials for Tumor Protein p53 (P53) | Main materials for "Do It (ELISA Kit) Yourself". |