High Sensitive ELISA Kit for Thymic Stromal Lymphopoietin (TSLP)

Specificity

This assay has high sensitivity and excellent specificity for detection of High Sensitive Thymic Stromal Lymphopoietin (TSLP).
No significant cross-reactivity or interference between High Sensitive Thymic Stromal Lymphopoietin (TSLP) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant High Sensitive Thymic Stromal Lymphopoietin (TSLP) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Thymic Stromal Lymphopoietin (TSLP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-98 87
EDTA plasma(n=5) 84-98 89
heparin plasma(n=5) 88-104 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Thymic Stromal Lymphopoietin (TSLP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Thymic Stromal Lymphopoietin (TSLP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Thymic Stromal Lymphopoietin (TSLP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-102% 93-101% 78-91% 81-105%
EDTA plasma(n=5) 81-99% 95-102% 81-93% 86-95%
heparin plasma(n=5) 93-102% 97-105% 78-91% 88-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Journal of Interferon & Cytokine Research Epithelial Cell-Derived Cytokines Contribute to the Pathophysiology of Eosinophilic Chronic Rhinosinusitis Pubmed:26540312
Allergology International The effect of calprotectin on TSLP and IL-25 production from airway epithelial cells pubmed:27475624
International Journal of Molecular Sciences Topical Application of JAK1/JAK2 Inhibitor Momelotinib Exhibits Significant Anti-Inflammatory Responses in DNCB-Induced Atopic Dermatitis Model Mice Pubmed: 30544712
Dermatol Reports Skin tissue expression and serum level of thymic stromal lymphopoietin in patients with psoriasis vulgaris Pubmed: 31316739
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