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ELISA Kit for Thymic Stromal Lymphopoietin (TSLP)

Specificity

This assay has high sensitivity and excellent specificity for detection of Thymic Stromal Lymphopoietin (TSLP).
No significant cross-reactivity or interference between Thymic Stromal Lymphopoietin (TSLP) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Thymic Stromal Lymphopoietin (TSLP) and the recovery rates were calculated by comparing the measured value to the expected amount of Thymic Stromal Lymphopoietin (TSLP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-97 93
EDTA plasma(n=5) 84-103 88
heparin plasma(n=5) 85-94 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thymic Stromal Lymphopoietin (TSLP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thymic Stromal Lymphopoietin (TSLP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Thymic Stromal Lymphopoietin (TSLP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-101% 95-102% 79-101% 82-98%
EDTA plasma(n=5) 96-103% 89-98% 78-93% 95-105%
heparin plasma(n=5) 91-98% 95-102% 90-104% 88-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Reference
Journal of Interferon & Cytokine Research Epithelial Cell-Derived Cytokines Contribute to the Pathophysiology of Eosinophilic Chronic Rhinosinusitis Pubmed:26540312
Allergology International The effect of calprotectin on TSLP and IL-25 production from airway epithelial cells pubmed:27475624
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB320Hu01 Recombinant Thymic Stromal Lymphopoietin (TSLP) Positive Control; Immunogen; SDS-PAGE; WB.
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PAB320Hu01 Polyclonal Antibody to Thymic Stromal Lymphopoietin (TSLP) WB; IHC; ICC; IP.
MAB320Hu22 Monoclonal Antibody to Thymic Stromal Lymphopoietin (TSLP) WB; IHC; ICC; IP.
SEB320Hu ELISA Kit for Thymic Stromal Lymphopoietin (TSLP) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB320Hu CLIA Kit for Thymic Stromal Lymphopoietin (TSLP) Chemiluminescent immunoassay for Antigen Detection.