ELISA Kit DIY Materials for Complement Component 5a (C5a)
- Product No.KSA388Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range1.56-100ng/mL
- Applicable Sensitivity0.57ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- UOM 96T*596T*10 96T*20 96T*50 96T*100
For more details, please contact local distributors! US$ 1728
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The Abs in the kit have high sensitivity and excellent specificity for detection of Complement Component 5a (C5a). No significant cross-reactivity or interference between Complement Component 5a (C5a) and analogues was observed.
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
|Molecular Immunology||Cathepsin D is released after severe tissue trauma in vivo and is capable of generating C5a in vitro ScienceDirect: S0161589011008297|
|Journal of Reproductive Immunology||Elevated complement factor C5a in maternal and umbilical cord plasma in preeclampsia PubMed: 23415845|
|The Journal of Clinical Investigation||Complement component 5 contributes to poor disease outcome in humans and mice with pneumococcal meningitis PubMed: PMC3195471|
|Journal of Antimicrobial Chemotherapy||Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model Pubmed: 23645587|
|Journal of Orthopaedic Research||Conversion from external fixator to intramedullary nail causes a second hit and impairs fracture healing in a severe trauma model Pubmed: 23070742|
|SpringerPlus||Therapeutic effects of sesame oil on monosodium urate crystal-induced acute inflammatory response in rats Pubmed: 24353977|
|American Journal fo Reproductive Immunology||Evaluation of the Markers of Inflammation in the Umbilical Cord Blood of Newborns of Mothers with Thrombophilia Pubmed:25209155|
|Gynecol Obstet Invest||Complement Split Products C3a/C5a and Receptors: Are They Regulated by Circulating Angiotensin II Type 1 Receptor Autoantibody in Severe Preeclampsia? PubMed: 26485247|
|Journal of Neuroimmunology||Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis Pubmed:27235358|
|International Immunopharmacology||Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery Pubmed:26802602|
|Digital Repository||Dosagem de frações ativadas do sistema complemento em empiema induzido em ratos 10183|
|Environmental Pollution||Cadmium-induced immune abnormality is a key pathogenic event in human and rat models of preeclampsia pubmed:27511439|
|Cancer Letters||Complement C5a/C5aR pathway potentiates the pathogenesis Q5 of gastric cancer by down-regulating p21 expression pubmed:29031586|
|Acta Paediatrica||Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese children DOI: 10.1111/apa.14106|
|Scientific Reports||Splenectomy modulates early immuno-inflammatory responses to trauma-hemorrhage and protects mice against secondary sepsis Pubmed: 30291296|
|Nutrients||The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in Triathletes Pubmed: 30736479|
|Journal of NeuroVirology||Intrathecal complement activation by the classical pathway in tick-borne encephalitis Pubmed: 30850976|
|PLoS One||Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model Pubmed: 31247004|
|Frontiers in Immunology||C5a/C5aR Pathway is Critical for the Pathogenesis of Psoriasis Pubmed: 31447855|
|Phytomedicine||Novel evidence that an alternative complement cascade pathway is involved in optimal mobilization of hematopoietic stem/progenitor cells in Nlrp3 …|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA388Mu01||Recombinant Complement Component 5a (C5a)||Positive Control; Immunogen; SDS-PAGE; WB.|
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|PAA388Mu01||Polyclonal Antibody to Complement Component 5a (C5a)||WB; IHC; ICC; IP.|
|LAA388Mu71||Biotin-Linked Polyclonal Antibody to Complement Component 5a (C5a)||WB; IHC; ICC.|
|MAA388Mu21||Monoclonal Antibody to Complement Component 5a (C5a)||WB; IHC; ICC; IP.|
|LAA388Mu72||Biotin-Linked Monoclonal Antibody to Complement Component 5a (C5a)||WB; IHC; ICC.|
|SEA388Mu||ELISA Kit for Complement Component 5a (C5a)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|HEA388Mu||High Sensitive ELISA Kit for Complement Component 5a (C5a)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA388Mu||CLIA Kit for Complement Component 5a (C5a)||Chemiluminescent immunoassay for Antigen Detection.|
|PSA388Mu01||Antibody Pair for Complement Component 5a (C5a)||ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.|
|KSA388Mu01||ELISA Kit DIY Materials for Complement Component 5a (C5a)||Main materials for "Do It (ELISA Kit) Yourself".|