ELISA Kit DIY Materials for Aspartate Aminotransferase (AST)

cCAT; AAT; ASAT; SGOT; GOT1; Cysteine transaminase, cytoplasmic; Transaminase A; Aspartate Transaminase 1,Cytoplasmic; Glutamic-Oxaloacetic Transaminase 1,Soluble

  • Product No.KSB214Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.781-50ng/mL
  • Applicable Sensitivity0.34ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1300  US$ 2166  US$ 3899  US$ 7581  US$ 12996 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Aspartate Aminotransferase (AST).No significant cross-reactivity or interference between Aspartate Aminotransferase (AST) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Kidney International Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and -independent mechanisms PubMed: PMC3612362
Proceedings of the National Academy of Sciences Pathogenesis of emerging severe fever with thrombocytopenia syndrome virus in C57/BL6 mouse model PubMed: PMC3382536
African Journal of Pharmacy and Pharmacology Protective effect of Panax ginseng against N-acetyl-p-aminophenol-induced hepatotoxicity in rats Academicjournals: Source
Kidney International Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and-independent mechanisms Pubmed: 23325074
European Journal of Pharmacology Protective effect of Et-1 receptor antagonist bosentan on paracetamol induced acute liver toxicity in rats ScienceDirect: S0014299914000235
Journal of Immunology The P2X1 Receptor Is Required for Neutrophil Extravasation during Lipopolysaccharide-Induced Lethal Endotoxemia in Mice Pubmed:25480563
Gastroenterol Res Pract Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol Poisoning PubMed: 26697061
Gastroenterol Res Pract. Advanced Studies in Clinical and Experimental Research in Gastroenterology Pubmed:26955389
Scientific Reports Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway. pubmed:27678302
 Biochimica et Biophysica Acta (BBA)-General Subjects Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in rats pubmed:28341485
Scientific Reports Deletion of MCP-1 Impedes Pathogenesis of Acid Ceramidase Deficiency Pubmed:29379059
FEBS Open Bio A critical role of hepatitis B virus polymerase in cirrhosis, hepatocellular carcinoma, and steatosis Pubmed:29321963
BMC Complementary and Alternative Medicine  Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituents Pubmed: 28095910
laboratory investigation Hepatic pathology and altered gene transcription in a murine model of acid ceramidase deficiency Pubmed: 31186526
Brain Behavior and Immunity Corticosterone-mediated microglia activation affects dendritic spine plasticity and motor learning functions in minimal hepatic encephalopathy Pubmed: 31437533
Biomed Research International Liraglutide Attenuates Nonalcoholic Fatty Liver Disease by Modulating Gut Microbiota in Rats Administered a High-Fat Diet Pubmed: 32149099
Nanomaterials Biological Safety and Biodistribution of Chitosan Nanoparticles Pubmed: 32340313
European Journal of Biology Effects of Tempol in Lipopolysaccharide-Induced Liver Injury
Pathogens Antimalarial Effect of the Total Glycosides of the Medicinal Plant, Ranunculus japonicus 33925018
Int Immunopharmacol Taraxasterol mitigates Con A-induced hepatitis in mice by suppressing interleukin-2 expression and its signaling in T lymphocytes 34848154
Acta Pharmacol Sin NFAT inhibitor 11R-VIVIT ameliorates mouse renal fibrosis after ischemia-reperfusion-induced acute kidney injury 34937917
J Magn Reson Imaging Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver … Pubmed:35212074
Medcomm Blockade of mIL‐6R alleviated lipopolysaccharide‐induced systemic inflammatory response syndrome by suppressing NF‐κB‐mediated Ccl2 expression and … Pubmed:35548710
International Immunopharmacology Dimethyl fumarate ameliorates autoimmune hepatitis in mice by blocking NLRP3 inflammasome activation Pubmed:35605433
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KSB214Ra01 ELISA Kit DIY Materials for Aspartate Aminotransferase (AST) Main materials for "Do It (ELISA Kit) Yourself".