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ELISA Kit DIY Materials for Fatty Acid Binding Protein 4 (FABP4)
A-FABP; AFABP; AP2; Fatty Acid Binding Protein 4, Adipocyte; Adipocyte Protein 2; Adipocyte-type fatty acid-binding protein
- Product No.KSB693Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range31.2-2000pg/mL
- Applicable Sensitivity13.8pg/mL
- ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
- Downloadn/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1231
US$ 2052
US$ 3694
US$ 7182
US$ 12312
For more details, please contact local distributors!
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Fatty Acid Binding Protein 4, Adipocyte (FABP4). No significant cross-reactivity or interference between Fatty Acid Binding Protein 4, Adipocyte (FABP4) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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| Magazine | Citations |
| Clinical and Molecular Hepatology | Enhanced A-FABP expression in visceral fat: potential contributor to the progression of NASH PubMed: 23091808 |
| journal of Human Hypertension | Siesta is associated with reduced systolic blood pressure level and decreased prevalence of hypertension in older adults PubMed: 26134622 |
| genetics and molecular research | Overexpression of the A-FABP gene facilitates intermuscular fat deposition in transgenic mice PubMed: 25867423 |
| Food Chem | Cranberries (Oxycoccus quadripetalus) inhibit lipid metabolism and modulate leptin and adiponectin secretion in 3T3-L1 adipocytes PubMed: 25952883 |
| Placenta | Increased expression of fatty acid binding protein 4 in preeclamptic Placenta and its relevance to preeclampsia Pubmed:26992681 |
| Clin Chim Acta. | Valproate acid (VPA)-induced dysmetabolic function in clinical and animal studies. pubmed:28161274 |
| Oxidative Medicine and Cellular Longevity | Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague … Pubmed:29849871 |
| Nutrition & Diabetes | Lack of pronounced changes in the expression of fatty acid handling proteins in adipose tissue and plasma of morbidly obese humans Pubmed:29335416 |
| American Journal of Cancer Research | 25-HC promotes hepatocellular carcinoma metastasis through up-regulation of TLR4 dependent FABP4 Pubmed: 31720079 |
| J Magn Reson Imaging | Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver … Pubmed:35212074 |
| cells | The Effect of Mineralocorticoid Receptor 3 Antagonists on Anti-Inflammatory and Anti-Fatty Acid Transport Profile in Patients with Heart Failure Pubmed:35455943 |
| Bone Research | FABP4 secreted by M1-polarized macrophages promotes synovitis and angiogenesis to exacerbate rheumatoid arthritis Pubmed:35729106 |