ELISA Kit DIY Materials for Glucose Transporter 4 (GLUT4)

SLC2A4; Solute carrier family 2, facilitated glucose transporter member 4; Glucose transporter type 4, insulin-responsive

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Glucose Transporter 4 (GLUT4). No significant cross-reactivity or interference between Glucose Transporter 4 (GLUT4) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Polar Record Glucose transporter-4 in white blood cells of young and old sled dogs: a model for human biomarker development Cambridge: Source
The International Journal of Biochemistry & Cell Biology Conditioning causes an increase in glucose transporter-4 levels in mononuclear cells in sled dogs Pubmed:25236492
Psychoneuroendocrinology Maternal stress predicts altered biogenesis and the profile of mitochondrial proteins in the frontal cortex and hippocampus of adult offspring rats PubMed: 26143539
PLOS ONE Antidiabetic Effect of Galantamine: Novel Effect for a Known Centrally Acting Drug PubMed: 26262991
American Journal of Animal and Veterinary Sciences Metabolic Features of Heart Failure with Different Etiology ofsp11075
Journal of Pharmaceutical Negative Results Hibiscus sabdariffa linn fruits methanolic extract and fractions mediated glucose uptake stimulation and glucose transporter 4 regulation Doi: 10.4103/jpnr.JPNR_14_18
eureka herba indonesia Effect of Noni Fruit Extract (Morinda citrifolia) on Glucose Intake to Diabetes Mellitus White Rat Muscle Tissue
eureka herba indonesia The Effects of Sambiloto Leaf Extract (Andrographis peniculata) on Blood Sugar Regulation: an In Vivo Study
eureka herba indonesia Effect of Bay Leaf Extract (Syzygium polyanthum) on Blood Sugar Regulation via GLUT4 Protein Regulation in White Rat Muscle Tissue Induced Aloxan
Eureka Herba Indonesia Potential Combination of Tinospora crispa, Andrographis paniculata, Cinamomum burmanii, Syzygium polyanthum and Momordica charantia Extracts Against?¡­
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