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ELISA Kit DIY Materials for Noggin (NOG)

SYM1; SYNS1; Synostoses(Multiple)Syndrome 1; Symphalangism 1(Proximal)

  • Product No.KSC130Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.66ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1296  US$ 2160  US$ 3888  US$ 7560  US$ 12960 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Noggin (NOG). No significant cross-reactivity or interference between Noggin (NOG) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Arthritis Rheumatol Imbalance between BMP2 and Noggin induces abnormal osteogenic differentiation of mesenchymal stem cells in ankylosing spondylitis PubMed: 26413886
Arthritis Rheumatol.  Imbalance Between Bone Morphogenetic Protein 2 and Noggin Induces Abnormal Osteogenic Differentiation of Mesenchymal Stem Cells in Ankylosing Spondylitis Pubmed:26413886
Clin Exp Pharmacol Physiol. Oxygen-glucose deprivation preconditioning protects neurons against oxygen-glucose deprivation/reperfusion induced injury via bone morphogenetic protein-7 mediated ERK, p38 and Smad signalling pathways. Pubmed:26385023
European cells & materials The BMP2 variant L51P restores the osteogenic differentiation of human mesenchymal stromal cells in the presence of intervertebral disc cells. pubmed:28266688
Arthritis & Rheumatology mbalance Between Bone Morphogenetic Protein 2 and Noggin Induces Abnormal OsteogenicDifferentiation of Mesenchymal Stem Cells in Ankylosing Spondylitis. pubmed:26413886
Biotechnology and Applied Biochemistry The BMP antagonist Noggin is produced by osteoblasts in response to the presence of prostate cancer cells. pubmed:28981962
Heliyon Efficient in vitro delivery of Noggin siRNA enhances osteoblastogenesis pubmed:29167826
Journal of Cellular Biochemistry Low‐intensity pulsed ultrasound promotes bone morphogenic protein 9‐induced osteogenesis and suppresses inhibitory effects of inflammatory cytokines on cellular … Pubmed: 31006911
Aging Male Effect of tadalafil on penile nitric oxide synthase and corporal smooth muscle in rats under dutasteride treatment Pubmed: 32160825
J Reconstr Microsurg Effect of Simvastatin Use in Free Tissue Transfer: An Experimental Study in a Rat Epigastric Free Flap Model Pubmed: 31995818
Biochem Biophys Rep Slow diffusion on the monolayer culture enhances auto/paracrine effects of Noggin in differentiation of human iPS cells induced by BMP Pubmed:35005256
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPC130Mu01 Recombinant Noggin (NOG) Positive Control; Immunogen; SDS-PAGE; WB.
RPC130Mu02 Recombinant Noggin (NOG) Positive Control; Immunogen; SDS-PAGE; WB.
PAC130Mu01 Polyclonal Antibody to Noggin (NOG) WB; IHC; ICC; IP.
PAC130Mu02 Polyclonal Antibody to Noggin (NOG) WB; IHC; ICC; IP.
SEC130Mu ELISA Kit for Noggin (NOG) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC130Mu Multiplex Assay Kit for Noggin (NOG) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSC130Mu01 ELISA Kit DIY Materials for Noggin (NOG) Main materials for "Do It (ELISA Kit) Yourself".