Multiplex Assay Kit for Beta Catenin (β-catenin) ,etc. by FLIA (Flow Luminescence Immunoassay)

CTNNb1; bCat; b-Cat; CTNN-B1; Catenin Beta 1; Cadherin-Associated Protein

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Beta Catenin (β-catenin) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Beta Catenin (β-catenin) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Beta Catenin (β-catenin) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Beta Catenin (β-catenin) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-97 94
EDTA plasma(n=5) 84-101 97
heparin plasma(n=5) 80-101 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta Catenin (β-catenin) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta Catenin (β-catenin) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Beta Catenin (β-catenin) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-98% 86-96% 80-94% 93-101%
EDTA plasma(n=5) 89-103% 80-101% 89-101% 79-101%
heparin plasma(n=5) 78-97% 97-105% 78-101% 80-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:β-catenin) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Clinica Chimica Acta Versican and its associated molecules: Potential diagnostic markers for multiple myeloma Pubmed:25623955
Journal of Bone and Mineral Research Adverse effects of osteocytic constitutive activation of ?-catenin on bone strength and bone growth Pubmed:25639729
Am J Transl Res Role of SDF-1 and Wnt signaling pathway in the myocardial fibrosis of hypertensive rats PubMed: 26396666
Scientific Reports Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA. pubmed:28533512
Life Sciences Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways. pubmed:28754618
Scientific Reports Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA Pubmed: 28533512
The Showa University Journal of Medical Sciences Eribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β-catenin Signaling Pathway in Triple-negative Breast Cancer Doi: 10.15369/sujms.30.359
Advances in Medical Sciences Urinary procollagen III aminoterminal propeptide and β-catenin–New diagnostic biomarkers in solitary functioning kidney? Pubmed: 30738287
Biomed Pharmacother Silence of FAM83H-AS1 promotes chemosensitivity of gastric cancer through Wnt/β-catenin signaling pathway Pubmed: 32028241
Journal of Cosmetic Dermatology Novel complex of cosmetic ingredients with promising action in preventing hair loss and follicular aging through mechanism involving enrichment of WNT/signaling … Pubmed: 33179848
Nutrients Hydroxytyrosol as a Promising Ally in the Treatment of Fibromyalgia Pubmed: 32784915
Oncotarget Amalgamation of PI3K and EZH2 blockade synergistically regulates invasion and angiogenesis: combination therapy for glioblastoma multiforme 33473259
Cytokine QNZ alleviated hepatocellular carcinoma by targeting inflammatory pathways in a rat model 34564023
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB021Mu02 Recombinant Beta Catenin (β-catenin) Positive Control; Immunogen; SDS-PAGE; WB.
RPB021Mu01 Recombinant Beta Catenin (β-catenin) Positive Control; Immunogen; SDS-PAGE; WB.
PAB021Mu02 Polyclonal Antibody to Beta Catenin (β-catenin) WB; IHC; ICC; IP.
PAB021Mu01 Polyclonal Antibody to Beta Catenin (β-catenin) WB; IHC; ICC; IP.
SEB021Mu ELISA Kit for Beta Catenin (β-catenin) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB021Mu Multiplex Assay Kit for Beta Catenin (β-catenin) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB021Mu01 ELISA Kit DIY Materials for Beta Catenin (β-catenin) Main materials for "Do It(ELISA Kit) Yourself"