Packages (Simulation)
Image (I)
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DAB staining on IHC-P;
Sample: Porcine Uterus Tissue
Primary Ab: 30µg/ml Mouse Anti-Rat CK4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Porcine Liver Tissue
Primary Ab: 30µg/ml Mouse Anti-Rat CK4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Porcine Lung Tissue
Primary Ab: 30µg/ml Mouse Anti-Rat CK4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Porcine Pancreas Tissue
Primary Ab: 30µg/ml Mouse Anti-Rat CK4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Image (II)
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Figure.Western Blot;Sample: Recombinant KRT4, Rat.
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Western Blot; Samples: Lane1: A431 cell lysate; Lane2: A549 cell lysate; Lane3: Hela cell lysate; Lane4: MCF7 cell lysate;
Primary Ab: 0.2µg/ml Mouse Anti-Rat CK4 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Quality Guarantee
Instruction Manual
Certificate
Monoclonal Antibody to Cytokeratin 4 (CK4)
CYK4; KRT4; K4; Keratin 4; Keratin, type II cytoskeletal 4; Type-II keratin Kb4
- Product No.MAA489Ra21
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)1:270000 (1#)
- Ig Isotype IgG1 Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPA489Ra01-Recombinant Cytokeratin 4 (CK4)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration1mg/mL
- Organism Species MoreHomo sapiens (Human), Sus scrofa; Porcine (Pig)
- ApplicationsWB; IHC
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 109
US$ 253
US$ 362
US$ 905
US$ 3620
For more details, please contact local distributors!
SPECIFITY
The antibody is a mouse monoclonal antibody raised against CK4. It has been selected for its ability to recognize CK4 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| Molecular Medicine Reports | Placental protein 14 as a potential biomarker for diagnosis of preterm premature rupture of membranes Pubmed:29749501 |
| FEBS Open Bio | Development of a novel murine heart failure model overexpressing human renin and angiotensinogen Pubmed: 32056390 |
| J Clin Periodontol | Discovery, validation, and diagnostic ability of multiple protein-based biomarkers in saliva and gingival crevicular fluid to distinguish between health and periodontal … Pubmed:35451104 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA489Ra01 | Recombinant Cytokeratin 4 (CK4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA489Ra01 | Polyclonal Antibody to Cytokeratin 4 (CK4) | WB; IHC |
| LAA489Ra71 | Biotin-Linked Polyclonal Antibody to Cytokeratin 4 (CK4) | WB; IHC; ICC. |
| MAA489Ra21 | Monoclonal Antibody to Cytokeratin 4 (CK4) | WB; IHC |