Mini Samples ELISA Kit for Gastric Inhibitory Polypeptide (GIP)

Incretin hormone; Glucose Dependent Insulinotropic Peptide

Specificity

This assay has high sensitivity and excellent specificity for detection of Mini Samples Gastric Inhibitory Polypeptide (GIP).
No significant cross-reactivity or interference between Mini Samples Gastric Inhibitory Polypeptide (GIP) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Gastric Inhibitory Polypeptide (GIP) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Gastric Inhibitory Polypeptide (GIP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 94-102 98
EDTA plasma(n=5) 90-105 94
heparin plasma(n=5) 82-97 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Gastric Inhibitory Polypeptide (GIP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Gastric Inhibitory Polypeptide (GIP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Gastric Inhibitory Polypeptide (GIP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 97-105% 92-99% 97-104% 81-96%
EDTA plasma(n=5) 87-104% 82-102% 86-93% 86-98%
heparin plasma(n=5) 89-98% 92-99% 93-101% 80-94%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×9mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well.
    And then add 25μL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 50µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 50µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 25µL Stop Solution. Read at 450 nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Scientific Reports The anti-hyperglycemic efficacy of a lipid-lowering drug Daming capsule and the underlyingsignaling mechanisms in a rat model of diabetes mellitus. pubmed:27721485
Obesity surgery The Effects of Duodenojejunal Omega Switch in Combination with High-Fat Diet and Control Diet on Incretins, Body Weight, and Glucose Tolerance in Sprague-Dawley Rats. pubmed:28840471
Obesity Surgery  Preserving Duodenal-Jejunal (Foregut) Transit Does Not Impair Glucose Tolerance and Diabetes Remission Following Gastric Bypass in Type 2 Diabetes Sprague … Pubmed:29098544
BioMed Research International Predictors of Effectiveness of Glucagon-Like Peptide-1 Receptor Agonist Therapy in Patients with Type 2 Diabetes and Obesity
Diabetes Research and Clinical Practice Low AS160 and high SGK basal phosphorylation associates with impaired incretin profile and type 2 diabetes in adipose tissue of obese patients Pubmed: 31734225
OBESITY SURGERY The Leading Role of Peptide Tyrosine Tyrosine in Glycemic Control After Roux-en-Y Gastric Bypass in Rats Pubmed: 31701411
FUTURE SCIENCE Prediction scale of response to liraglutide therapy as the method for increase of treatment efficacy in type 2 diabetes Pubmed:35251693
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA882Ra01 Recombinant Gastric Inhibitory Polypeptide (GIP) Positive Control; Immunogen; SDS-PAGE; WB.
CPA882Ra11 BSA Conjugated Gastric Inhibitory Polypeptide (GIP) Immunogen; SDS-PAGE; WB.
CPA882Ra21 OVA Conjugated Gastric Inhibitory Polypeptide (GIP) Immunogen; SDS-PAGE; WB.
PAA882Ra08 Polyclonal Antibody to Gastric Inhibitory Polypeptide (GIP) WB; IHC; ICC; IP.
PAA882Ra01 Polyclonal Antibody to Gastric Inhibitory Polypeptide (GIP) WB; IHC; ICC; IP.
MAA882Ra21 Monoclonal Antibody to Gastric Inhibitory Polypeptide (GIP) WB; IHC; ICC; IP.
MEA882Ra Mini Samples ELISA Kit for Gastric Inhibitory Polypeptide (GIP) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA882Ra ELISA Kit for Gastric Inhibitory Polypeptide (GIP) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA882Ra Multiplex Assay Kit for Gastric Inhibitory Polypeptide (GIP) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA882Ra11 ELISA Kit DIY Materials for Gastric Inhibitory Polypeptide (GIP) Main materials for "Do It (ELISA Kit) Yourself".