Mini Samples ELISA Kit for Peptidase D (PEPD)

PRD; Xaa-Pro Dipeptidase; Prolidase; Imidodipeptidase; Proline dipeptidase

Specificity

This assay has high sensitivity and excellent specificity for detection of Mini Samples Peptidase D (PEPD).
No significant cross-reactivity or interference between Mini Samples Peptidase D (PEPD) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Peptidase D (PEPD) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Peptidase D (PEPD) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×4.5mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPM011Hu01 Recombinant Peptidase D (PEPD) Positive Control; Immunogen; SDS-PAGE; WB.
PAM011Hu01 Polyclonal Antibody to Peptidase D (PEPD) WB; IHC; ICC; IP.
MAM011Hu21 Monoclonal Antibody to Peptidase D (PEPD) WB; IHC; ICC; IP.
MEM011Hu Mini Samples ELISA Kit for Peptidase D (PEPD) Enzyme-linked immunosorbent assay for Antigen Detection.
SEM011Hu ELISA Kit for Peptidase D (PEPD) Enzyme-linked immunosorbent assay for Antigen Detection.