Packages (Simulation)

Polyclonal Antibody to Focal Adhesion Kinase (FAK)

Image (I)

DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human FAK Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Thyroid Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human FAK Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human FAK Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human FAK Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human FAK Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant FAK, Human.
Western Blot; Sample: Human Lung lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human FAK Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: K562 cell lysate; Lane2: A549 cell lysate;
Primary Ab: 0.2µg/ml Rabbit Anti-Human FAK Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human K562 cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human FAK Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Jurkat cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human FAK Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: Rat Cerebrum lysate; Lane2: RAW264.7 cell lysate;
Primary Ab: 0.2µg/ml Rabbit Anti-Human FAK Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Focal-Adhesion-Kinase-(FAK)-PAB333Hu01.pdf

Certificate

Polyclonal Antibody to Focal Adhesion Kinase (FAK)

PTK2; FADK; FAK1; Pp125FAK; PPP1R71; Protein Tyrosine Kinase 2; Focal adhesion kinase-related nonkinase; Protein phosphatase 1 regulatory subunit 71

Product No.PAB333Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB333Hu01-Recombinant Focal Adhesion Kinase (FAK)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species Moren/a
ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 94 220 US$ 220 US$ 314 US$ 785 US$ 3140
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against FAK. It has been selected for its ability to recognize FAK in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Clinica Chimica Acta	Versican and its associated molecules: Potential diagnostic markers for multiple myeloma Pubmed:25623955

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPB333Hu01	Recombinant Focal Adhesion Kinase (FAK)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB333Hu01	Polyclonal Antibody to Focal Adhesion Kinase (FAK)	WB,IHC
MAB333Hu21	Monoclonal Antibody to Focal Adhesion Kinase (FAK)	WB; IHC; ICC; IP.
MAB333Hu22	Monoclonal Antibody to Focal Adhesion Kinase (FAK)	WB; IHC; ICC; IP.
MAB333Hu23	Monoclonal Antibody to Focal Adhesion Kinase (FAK)	WB; IHC; ICC; IP.
MAB333Hu24	Monoclonal Antibody to Focal Adhesion Kinase (FAK)	WB; IHC; ICC; IP.
SEB333Hu	ELISA Kit for Focal Adhesion Kinase (FAK)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB333Hu	Multiplex Assay Kit for Focal Adhesion Kinase (FAK) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.