Packages (Simulation)

Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT)

Image (I)

DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human MGMT Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human MGMT Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Testis Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human MGMT Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Liver Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human MGMT Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Kidney Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human MGMT Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant MGMT, Human.
Western Blot; Sample: Jurkat cell lysate
Primary Ab: 3µg/ml Rabbit Anti-Human MGMT Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type Jurkat cell lysate;
Lane 2: MGMT knockout Jurkat cell lysate;
Predicted MW: 21kd
Observed MW: 22kd
Primary Ab: 3µg/ml Rabbit Anti-Human MGMT Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-O-6-Methylguanine-DNA-Methyltransferase-(MGMT)-PAC027Hu01.pdf

Certificate

KO-Validated

Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT)

Methylated-DNA--Protein-Cysteine Methyltransferase; 6-O-methylguanine-DNA methyltransferase; Methylated-DNA--protein-cysteine methyltransferase

Product No.PAC027Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPC027Hu01-Recombinant O-6-Methylguanine DNA Methyltransferase (MGMT)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species Moren/a
ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against MGMT. It has been selected for its ability to recognize MGMT in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Biotechnology and Bioengineering	Targeted nitric oxide delivery preferentially induces glioma cell chemosensitivity via altered p53 and O(6) -Methylguanine-DNA Methyltransferase activity PubMed: 23125026

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPC027Hu01	Recombinant O-6-Methylguanine DNA Methyltransferase (MGMT)	Positive Control; Immunogen; SDS-PAGE; WB.
PAC027Hu01	Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT)	WB,IHC
MAC027Hu22	Monoclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT)	WB; IHC; ICC; IP.
SEC027Hu	ELISA Kit for O-6-Methylguanine DNA Methyltransferase (MGMT)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMC027Hu	Multiplex Assay Kit for O-6-Methylguanine DNA Methyltransferase (MGMT) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.