Polyclonal Antibody to Insulin Receptor Substrate 1 (IRS1)

HIRS1

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against IRS1. It has been selected for its ability to recognize IRS1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
МЕДИЦИНСКИЙ ВЕСТНИК СЕВЕРНОГО КАВКАЗА Сравнительный анализ уровней антител к инсулиновым рецепторам у детей с сахарным диабетом i типа в зависимости от стадии заболевания article:n
Eur Neuropsychopharmacol. Regulation of insulin receptor phosphorylation in the brains of prenatally stressed rats: New insight into the benefits of antidepressant drug treatment. pubmed:28063625
Diabetes Lack of brain insulin receptor substrate-1 causes growth retardation, with decreased expression of growth hormone-releasing hormone in the hypothalamus. 33980693
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