Packages (Simulation)

Polyclonal Antibody to RAR Related Orphan Receptor Alpha (RORa)

Image (I)

IHC Images

Image (II)

Figure. Western Blot; Sample: Recombinant RORa, Human.
Western Blot; Sample: Lane1: PC3 cell lysate; Lane2: Jurkat cell lysate; Lane3: Mouse Kidney lysate
Primary Ab: 1µg/ml Rabbit Anti-Human RORa Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type PC3 cell lysate;
Lane 2: RORa knockout PC3 cell lysate;
Predicted MW: 63kd
Observed MW: 54kd
Primary Ab: 1µg/ml Rabbit Anti-Human RORa Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-RAR-Related-Orphan-Receptor-Alpha-(RORa)-PAD947Hu01.pdf

Certificate

KO-Validated

Polyclonal Antibody to RAR Related Orphan Receptor Alpha (RORa)

ROR1; ROR2; NR1F1; ROR3; RZRA; Nuclear receptor subfamily 1 group F member 1; Retinoid-related orphan receptor-alpha

Product No.PAD947Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPD947Hu01-Recombinant RAR Related Orphan Receptor Alpha (RORa)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species MoreMus musculus (Mouse)
ApplicationsWB
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against RORa. It has been selected for its ability to recognize RORa in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Biochemical genetics	Comparison of Selected Protein Levels in Tumour and Surgical Margin in a Group of Patients with Oral Cavity Cancer pubmed:28421310
neuroscience	Global knockdown of retinoid-related orphan receptor α in mature Purkinje cells reveals aberrant cerebellar phenotypes of spinocerebellar ataxia Pubmed: 32278059

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPD947Hu01	Recombinant RAR Related Orphan Receptor Alpha (RORa)	Positive Control; Immunogen; SDS-PAGE; WB.
PAD947Hu01	Polyclonal Antibody to RAR Related Orphan Receptor Alpha (RORa)	WB
SED947Hu	ELISA Kit for RAR Related Orphan Receptor Alpha (RORa)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMD947Hu	Multiplex Assay Kit for RAR Related Orphan Receptor Alpha (RORa) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.