Packages (Simulation)

Polyclonal Antibody to Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A)

Image (I)

DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human INPP4A Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Lymph node Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human INPP4A Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Cardiac Muscle Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human INPP4A Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Lymph node Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human INPP4A Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant INPP4A, Human.
Western Blot; Sample: Human Jurkat cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human INPP4A Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type MCF7 cell lysate;
Lane 2: INPP4A knockout MCF7 cell lysate;
Predicted MW: 106~110kd
Observed MW: 100kd
Primary Ab: 1µg/ml Rabbit Anti-Human INPP4A Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human A549 cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human INPP4A Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human MCF7 cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human INPP4A Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Inositol-Polyphosphate-4-Phosphatase-Type-I-107kDa-(INPP4A)-PAL624Hu01.pdf

Certificate

KO-Validated

Polyclonal Antibody to Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A)

INPP4; Inositol polyphosphate 4-phosphatase type I; Type I inositol 3,4-bisphosphate 4-phosphatase

Product No.PAL624Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPL624Hu01-Recombinant Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species Moren/a
ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 116 270 US$ 270 US$ 386 US$ 965 US$ 3860
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against INPP4A. It has been selected for its ability to recognize INPP4A in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Nature Communications	Loss-of-function of inositol polyphosphate-4-phosphatase reversibly increases the severity of allergic airway inflammation PubMed: 22673904
American Journal of Respiratory Cell and Molecular Biology	Secretory Inositol Polyphosphate 4-Phosphatase Protects Against Airway Inflammation and Remodeling Pubmed: 30335467

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPL624Hu01	Recombinant Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A)	Positive Control; Immunogen; SDS-PAGE; WB.
PAL624Hu01	Polyclonal Antibody to Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A)	WB,IHC
MAL624Hu21	Monoclonal Antibody to Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A)	WB; IHC; ICC; IP.
SEL624Hu	ELISA Kit for Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A)	Enzyme-linked immunosorbent assay for Antigen Detection.
LML624Hu	Multiplex Assay Kit for Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.