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Recombinant Cytochrome P450 7A1 (CYP7A1)

CYP7; CYP7-A1; CYPVII; Cholesterol 7 Alpha Hydroxylase; Cytochrome P450 Family 7 Subfamily A Polypeptide 1

  • Product No.RPB053Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane, Endoplasmic reticulum lumen
  • Predicted Molecular Mass34.4kDa
  • Accurate Molecular Mass38kDa(Analysis of differences refer to the manual)
  • Residues & TagsPhe109~Lys383 with N-terminal His Tag
  • Buffer Formulation100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 97%
  • Isoelectric Point6.9
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 187  US$ 468  US$ 936  US$ 2808  US$ 7020 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Cell Reports Parabacteroides distasonis Alleviates Obesity and Metabolic Dysfunctions via Production of Succinate and Secondary Bile Acids Pubmed: 30605678
Journal of Functional Foods Hyperglycemia exacerbates dyslipidemia-induced changes in uptake, synthesis, and transporters of bile acids in rats: Assessment of restorative potentials of ALA and?… Doi: 10.1016/j.jff.2019.01.038
Journal of Agriculture and Food Research Withaferin-A down-regulate enterohepatic circulation of bile acids: An insight from a hyperlipidemic rat model
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB053Ra01 Recombinant Cytochrome P450 7A1 (CYP7A1) Positive Control; Immunogen; SDS-PAGE; WB.
PAB053Ra01 Polyclonal Antibody to Cytochrome P450 7A1 (CYP7A1) WB; IHC; ICC; IP.
MAB053Ra21 Monoclonal Antibody to Cytochrome P450 7A1 (CYP7A1) WB; IHC; ICC; IP.
SEB053Ra ELISA Kit for Cytochrome P450 7A1 (CYP7A1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB053Ra Multiplex Assay Kit for Cytochrome P450 7A1 (CYP7A1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.