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Recombinant Acid Sphingomyelinase (ASM)

SMPD1; NPD; SMase; aSMase; Sphingomyelin Phosphodiesterase 1,Acid Lysosomal; Simply Sphingomyelinase

  • Product No.RPB360Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationLysosome
  • Predicted Molecular Mass33.8kDa
  • Accurate Molecular Mass30kDa(Analysis of differences refer to the manual)
  • Residues & TagsHis321~Pro581 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 95%
  • Isoelectric Point6.6
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 160  US$ 400  US$ 800  US$ 2400  US$ 6000 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Blood.? Acid sphingomyelinase is activated in sickle cell erythrocytes and contributes to inflammatory microparticle generation in SCD Pubmed:25075126
Journal of Clinical Lipidology Increase in acid sphingomyelinase level in human retinal endothelial cells and CD34+ circulating angiogenic cells isolated from diabetic individuals is associated with dysfunctional retinal vasculature and vascular repair process in diabetes 10.1016/j.jacl.2017.03.007
Diabetes & metabolism Fenofibrate decreases plasma ceramide in type 2 diabetes patients: A novel marker of CVD? pubmed:28499696
INFLAMMATION Acid Sphingomyelinase and Acid ¦Â-Glucosidase 1 Exert Opposite Effects on Interleukin-1¦Â-Induced Interleukin 6 Production in Rheumatoid Arthritis Fibroblast-Like Synoviocytes 33665756
Indian J Ophthalmol Evaluation of sphingolipid metabolism on diabetic retinopathy 34708809
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
EPB360Hu61 Eukaryotic Acid Sphingomyelinase (ASM) Positive Control; Immunogen; SDS-PAGE; WB.
RPB360Hu01 Recombinant Acid Sphingomyelinase (ASM) Positive Control; Immunogen; SDS-PAGE; WB.
APB360Hu61 Active Acid Sphingomyelinase (ASM) Cell culture; Activity Assays.
PAB360Hu01 Polyclonal Antibody to Acid Sphingomyelinase (ASM) WB; IHC; ICC; IP.
MAB360Hu22 Monoclonal Antibody to Acid Sphingomyelinase (ASM) WB; IHC; ICC; IP.
SEB360Hu ELISA Kit for Acid Sphingomyelinase (ASM) Enzyme-linked immunosorbent assay for Antigen Detection.
MEB360Hu Mini Samples ELISA Kit for Acid Sphingomyelinase (ASM) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB360Hu Multiplex Assay Kit for Acid Sphingomyelinase (ASM) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.