ELISA Kit for Acid Sphingomyelinase (ASM)

SMPD1; NPD; SMase; aSMase; Sphingomyelin Phosphodiesterase 1,Acid Lysosomal; Simply Sphingomyelinase

Specificity

This assay has high sensitivity and excellent specificity for detection of Acid Sphingomyelinase (ASM).
No significant cross-reactivity or interference between Acid Sphingomyelinase (ASM) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Acid Sphingomyelinase (ASM) and the recovery rates were calculated by comparing the measured value to the expected amount of Acid Sphingomyelinase (ASM) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-104 101
EDTA plasma(n=5) 97-104 101
heparin plasma(n=5) 78-104 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acid Sphingomyelinase (ASM) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acid Sphingomyelinase (ASM) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Acid Sphingomyelinase (ASM) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-105% 82-99% 80-96% 97-104%
EDTA plasma(n=5) 91-101% 86-101% 87-102% 88-102%
heparin plasma(n=5) 95-103% 89-102% 83-101% 84-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Blood.? Acid sphingomyelinase is activated in sickle cell erythrocytes and contributes to inflammatory microparticle generation in SCD Pubmed:25075126
Journal of Clinical Lipidology Increase in acid sphingomyelinase level in human retinal endothelial cells and CD34+ circulating angiogenic cells isolated from diabetic individuals is associated with dysfunctional retinal vasculature and vascular repair process in diabetes 10.1016/j.jacl.2017.03.007
Diabetes & metabolism Fenofibrate decreases plasma ceramide in type 2 diabetes patients: A novel marker of CVD? pubmed:28499696
INFLAMMATION Acid Sphingomyelinase and Acid ¦Â-Glucosidase 1 Exert Opposite Effects on Interleukin-1¦Â-Induced Interleukin 6 Production in Rheumatoid Arthritis Fibroblast-Like Synoviocytes 33665756
Indian J Ophthalmol Evaluation of sphingolipid metabolism on diabetic retinopathy 34708809
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