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Recombinant Topoisomerase I (TOP1)

TOPI; DNA Topoisomerase 1

  • Product No.RPC243Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationNucleus
  • Predicted Molecular Mass33.9kDa
  • Accurate Molecular Mass38kDa(Analysis of differences refer to the manual)
  • Residues & TagsMet1~Ser250 with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder
  • Purity> 80%
  • Isoelectric Point9.4
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 180  US$ 450  US$ 900  US$ 2700  US$ 6750 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Electronic Journal of Biotechnology Comparing the expression of human DNA topoisomerase I in KM71H and X33 strains of Pichia pastoris science:S0717345816000233
Journal of Experimental & Clinical Cancer Research Inhibitory effects of lapachol on rat C6 glioma in vitro and in vivo by targeting DNA topoisomerase I and topoisomerase II pubmed:27852319
Tikrit Journal of Pure Science Partial purification of topoisomerase IB from serum of diabetic patients and study it's kinetic properties and molecular weight
EurAsian Journal of BioSciences Study of topoisomerase I (Topo I) level and some biochemical parameters in diabetic patients
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC243Hu01 Recombinant Topoisomerase I (TOP1) Positive Control; Immunogen; SDS-PAGE; WB.
PAC243Hu01 Polyclonal Antibody to Topoisomerase I (TOP1) WB; IHC; ICC; IP.
MAC243Hu22 Monoclonal Antibody to Topoisomerase I (TOP1) WB; IHC; ICC; IP.
SEC243Hu ELISA Kit for Topoisomerase I (TOP1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC243Hu Multiplex Assay Kit for Topoisomerase I (TOP1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.