Packages (Simulation)

Polyclonal Antibody to Topoisomerase I (TOP1)

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Western Blot; Sample: Human MCF7 cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human TOP1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Placenta lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human TOP1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human TOP1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Topoisomerase-I-(TOP1)-PAC243Hu01.pdf

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Polyclonal Antibody to Topoisomerase I (TOP1)

TOPI; DNA Topoisomerase 1

Product No.PAC243Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPC243Hu01-Recombinant Topoisomerase I (TOP1)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species Moren/a
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against TOP1. It has been selected for its ability to recognize TOP1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Electronic Journal of Biotechnology	Comparing the expression of human DNA topoisomerase I in KM71H and X33 strains of Pichia pastoris science:S0717345816000233
Journal of Experimental & Clinical Cancer Research	Inhibitory effects of lapachol on rat C6 glioma in vitro and in vivo by targeting DNA topoisomerase I and topoisomerase II pubmed:27852319
Tikrit Journal of Pure Science	Partial purification of topoisomerase IB from serum of diabetic patients and study it's kinetic properties and molecular weight
EurAsian Journal of BioSciences	Study of topoisomerase I (Topo I) level and some biochemical parameters in diabetic patients

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPC243Hu01	Recombinant Topoisomerase I (TOP1)	Positive Control; Immunogen; SDS-PAGE; WB.
PAC243Hu01	Polyclonal Antibody to Topoisomerase I (TOP1)	WB; IHC; ICC; IP.
MAC243Hu22	Monoclonal Antibody to Topoisomerase I (TOP1)	WB; IHC; ICC; IP.
SEC243Hu	ELISA Kit for Topoisomerase I (TOP1)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMC243Hu	Multiplex Assay Kit for Topoisomerase I (TOP1) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.