CLIA Kit for Angiopoietin 2 (ANGPT2)
- Product No.SCA009Rb
- Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.062ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Angiopoietin 2 (ANGPT2).
No significant cross-reactivity or interference between Angiopoietin 2 (ANGPT2) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Angiopoietin 2 (ANGPT2) and the recovery rates were calculated by comparing the measured value to the expected amount of Angiopoietin 2 (ANGPT2) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiopoietin 2 (ANGPT2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiopoietin 2 (ANGPT2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Angiopoietin 2 (ANGPT2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Substrate A||1×10mL||Substrate B||1×2mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
|Microvascular Research||Mutant hypoxia inducible factor-1α improves angiogenesis and tissue perfusion in ischemic rabbit skeletal muscle PubMed: 20937289|
|Theriogenology||Evaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in Ewes Science: Article|
|Pediatr Infect Dis J||Important of Angiopoietic System in Evaluation of Endothelial Damage in Children with Crimean–Congo Hemorrhagic Fever PubMed: 25831422|
|Oncotarget||Long noncoding RNA hotair mediated angiogenesis in nasopharyngeal carcinoma by direct and indirect signaling pathways Pubmed:26717040|
|Experimental Animals||Ginsenoside Rb1 improves cardiac function and remodeling in heart failure. pubmed:28367863|
|Sci Rep.||Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis. pubmed:28276491|
|PeerJ||The ratio of serum Angiopoietin-1 to Angiopoietin-2 in patients with cervical cancer is a valuable diagnostic and prognostic biomarker pubmed:28584715|
|Cellular Physiology and Biochemistry||Angiotensin-(1-7) in Paraventricular Nucleus Contributes to the Enhanced Cardiac Sympathetic Afferent Reflex and Sympathetic Activity in Chronic Heart Failure Rats pubmed:28848201|
|Tumor Biology||Cervical cancer cell–derived angiopoietins promote tumor progression pubmed:28720059|
|Anesthesiology||Vasculotide, an angiopoietin-1 mimetic, restores microcirculatory perfusion and microvascular leakage and decreases fluid resuscitation requirements in … Pubmed:28968277|
|British Journal of Anaesthesia||Vasculotide, an angiopoietin-1 mimetic, reduces pulmonary vascular leakage and preserves microcirculatory perfusion during cardiopulmonary bypass in rats 10.1016:j.bja.2018.05.049|
|Anticancer Research||Oligodendrocytes Up-regulate the Invasive Activity of Glioblastoma Cells via the Angiopoietin-2 Signaling Pathway Pubmed: 30711932|
|Comparative Medicine||Characterization of a Porcine Model for Von Willebrand Disease Type 1 and 3 Regarding Expression of Angiogenic Mediators in the Nonpregnant Female … Pubmed: 31526432|
|BMC Endocrine Disorders||The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children Pubmed: 31771561|
|Biology of Reproduction||Expression of angiogenic factors in the uteroplacental unit is altered at time of placentation in a porcine model of von Willebrand disease type 1 Pubmed: 31806575|
|Scientific Reports||Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea Pubmed: 31882827|
|MEDIATORS OF INFLAMMATION||Mica Can Alleviate TNBS-Induced Colitis in Mice by Reducing Angiotensin II and IL-17A and Increasing Angiotensin-Converting Enzyme 2, Angiotensin 1-7 … Pubmed: 33100902|
|Scientific Reports||Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in rats Pubmed: 32616735|
|Catalog No.||Related products for research use of Oryctolagus cuniculus (Rabbit) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA009Rb01||Recombinant Angiopoietin 2 (ANGPT2)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA009Rb51||Polyclonal Antibody to Angiopoietin 2 (ANGPT2)||WB; IHC; ICC; IP.|
|MAA009Rb21||Monoclonal Antibody to Angiopoietin 2 (ANGPT2)||WB; IHC; ICC; IP.|
|SEA009Rb||ELISA Kit for Angiopoietin 2 (ANGPT2)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA009Rb||CLIA Kit for Angiopoietin 2 (ANGPT2)||Chemiluminescent immunoassay for Antigen Detection.|