ELISA Kit for Bone Morphogenetic Protein 2 (BMP2)
BMP2A; BMP-2A; Hemochromatosis Modifier
- Product No.SEA013Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.8pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 630
For more details, please contact local distributors! US$ 2835
For more details, please contact local distributors! US$ 5355
For more details, please contact local distributors! US$ 44100
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 2 (BMP2).
No significant cross-reactivity or interference between Bone Morphogenetic Protein 2 (BMP2) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Bone Morphogenetic Protein 2 (BMP2) and the recovery rates were calculated by comparing the measured value to the expected amount of Bone Morphogenetic Protein 2 (BMP2) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 2 (BMP2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 2 (BMP2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bone Morphogenetic Protein 2 (BMP2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Journal of Applied Toxicology||Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in mice Wiley: Source|
|PLoS ONE||A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source|
|Medical Journal of Chinese People's Liberation Army||Effect of transplantation of BMP-2-induced bone marrow mesenchymal stem cells on myocardial infarction of rats after reperfusion Source|
|PLOS ONE||PDGF-AA promotes osteogenic differentiation and migration of mesenchymal stem cell by down-regulating PDGFRα and derepressing BMP-Smad1/5/8 signaling Pubmed:25470749|
|Eur J Pharmacol||Negative effect of serotonin–norepinephrine reuptake inhibitor therapy on rat bone tissue after orchidectomy PubMed: 25934570|
|Pharmacology||Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy PubMed: 25871861|
|Journal of Cellular Biochemistry||Titanium with Nanotopography Induces Osteoblast Differentiation by Regulating Endogenous Bone Morphogenetic Protein Expression and Signaling Pathway PubMed: 26681207|
|International Journal of Nanomedicine||Sustained dual release of placental growth factor-2 and bone morphogenic protein-2 from heparin-based nanocomplexes for direct osteogenesis Pubmed:27042064|
|Research report||EFFECTS OF FLUORIDE ON THE EXPRESSION OF BMP-2 AND SMAD1 IN RAT OSTEOBLASTS IN VITRO files:p013-022|
|Gene||Unveiling the interactions among BMPR-2, ALK-1 and 5-HTT genes in the pathophysiology of HAPE Pubmed:27196063|
|Journal of Materials Chemistry B||Extracellular Matrix Powder from Cultured Cartilage-Like Tissue as Cell Carriers for Cartilage Repair DOI:10.1039/C7TB00640C|
|Scientific Reports||3D- Printed Poly(ε-caprolactone) Scaffold Integrated with Cell-laden Chitosan Hydrogels for Bone Tissue Engineering. pubmed:29042614|
|Journal of Applied Biomaterials & Functional Materials||Demineralized dentin and enamel matrices as suitable substrates for bone regeneration pubmed:28731486|
|Journal of Clinical Periodontology||Peroxisome proliferator‐activated receptor γ plays dual roles on experimental periodontitis in rats Pubmed:29574908|
|Pharmacology||Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats Pubmed:29898457|
|Journal of Applied Biomaterials & Functional Materials||BMP-2 and type I collagen preservation in human deciduous teeth after demineralization Pubmed:30045659|
|Applied Surface Science||Enhanced osteogenic differentiation of MC3T3-E1 on rhBMP-2 immobilized titanium surface through polymer-mediated electrostatic interaction|
|Materials Science and Engineering: C||Injectable hydrogels from enzyme-catalyzed crosslinking as BMSCs-laden scaffold for bone repair and regeneration|
|Biomaterials Science||Development of CaCO 3 microsphere-based composite hydrogel for dual delivery of growth factor and Ca to enhance bone regeneration|
|ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY||The dose-time effects of fluoride on the expression and DNA methylation level of the promoter region of BMP-2 and BMP-7 in rats Pubmed: 32004919|
|Journal of Industrial and Engineering Chemistry||Biofabrication and application of decellularized bone extracellular matrix for effective bone regeneration|
|BMC Musculoskeletal Disorders||Synergistic Effect of Platelet-rich Fibrin Releasate and Bone Marrow Stem Cells in Preventing Bone Loss in Ovariectomized Mouse|
|Bioact Mater||New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density 33842739|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|APA013Hu01||Active Bone Morphogenetic Protein 2 (BMP2)||Cell culture; Activity Assays.|
|RPA013Hu02||Recombinant Bone Morphogenetic Protein 2 (BMP2)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA013Hu01||Recombinant Bone Morphogenetic Protein 2 (BMP2)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA013Hu02||Active Bone Morphogenetic Protein 2 (BMP2)||Cell culture; Activity Assays.|
|PAA013Hu02||Polyclonal Antibody to Bone Morphogenetic Protein 2 (BMP2)||WB; IHC; ICC; IP.|
|PAA013Hu01||Polyclonal Antibody to Bone Morphogenetic Protein 2 (BMP2)||WB; IHC; ICC; IP.|
|MAA013Hu22||Monoclonal Antibody to Bone Morphogenetic Protein 2 (BMP2)||WB; IHC; ICC; IP.|
|SEA013Hu||ELISA Kit for Bone Morphogenetic Protein 2 (BMP2)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA013Hu||CLIA Kit for Bone Morphogenetic Protein 2 (BMP2)||Chemiluminescent immunoassay for Antigen Detection.|
|KSA013Hu01||ELISA Kit DIY Materials for Bone Morphogenetic Protein 2 (BMP2)||Main materials for "Do It(ELISA Kit) Yourself"|