ELISA Kit for Bone Morphogenetic Protein 2 (BMP2)

BMP2A; BMP-2A; Hemochromatosis Modifier

Specificity

This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 2 (BMP2).
No significant cross-reactivity or interference between Bone Morphogenetic Protein 2 (BMP2) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Bone Morphogenetic Protein 2 (BMP2) and the recovery rates were calculated by comparing the measured value to the expected amount of Bone Morphogenetic Protein 2 (BMP2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-102 99
EDTA plasma(n=5) 87-105 101
heparin plasma(n=5) 83-91 86

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 2 (BMP2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 2 (BMP2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bone Morphogenetic Protein 2 (BMP2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 97-105% 82-92% 89-97% 79-97%
EDTA plasma(n=5) 80-95% 94-101% 91-105% 82-104%
heparin plasma(n=5) 81-101% 82-90% 87-95% 94-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Journal of Applied Toxicology Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in mice Wiley: Source
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source
Medical Journal of Chinese People&#039;s Liberation Army Effect of transplantation of BMP-2-induced bone marrow mesenchymal stem cells on myocardial infarction of rats after reperfusion Source
PLOS ONE PDGF-AA promotes osteogenic differentiation and migration of mesenchymal stem cell by down-regulating PDGFRα and derepressing BMP-Smad1/5/8 signaling Pubmed:25470749
Eur J Pharmacol Negative effect of serotonin–norepinephrine reuptake inhibitor therapy on rat bone tissue after orchidectomy PubMed: 25934570
Pharmacology Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy PubMed: 25871861
Journal of Cellular Biochemistry Titanium with Nanotopography Induces Osteoblast Differentiation by Regulating Endogenous Bone Morphogenetic Protein Expression and Signaling Pathway PubMed: 26681207
International Journal of Nanomedicine Sustained dual release of placental growth factor-2 and bone morphogenic protein-2 from heparin-based nanocomplexes for direct osteogenesis Pubmed:27042064
Research report EFFECTS OF FLUORIDE ON THE EXPRESSION OF BMP-2 AND SMAD1 IN RAT OSTEOBLASTS IN VITRO files:p013-022
Gene  Unveiling the interactions among BMPR-2, ALK-1 and 5-HTT genes in the pathophysiology of HAPE Pubmed:27196063
Journal of Materials Chemistry B Extracellular Matrix Powder from Cultured Cartilage-Like Tissue as Cell Carriers for Cartilage Repair DOI:10.1039/C7TB00640C
Scientific Reports 3D- Printed Poly(ε-caprolactone) Scaffold Integrated with Cell-laden Chitosan Hydrogels for Bone Tissue Engineering. pubmed:29042614
Journal of Applied Biomaterials & Functional Materials Demineralized dentin and enamel matrices as suitable substrates for bone regeneration pubmed:28731486
Journal of Clinical Periodontology  Peroxisome proliferator‐activated receptor γ plays dual roles on experimental periodontitis in rats Pubmed:29574908
Pharmacology Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats Pubmed:29898457
Journal of Applied Biomaterials & Functional Materials BMP-2 and type I collagen preservation in human deciduous teeth after demineralization Pubmed:30045659
Applied Surface Science Enhanced osteogenic differentiation of MC3T3-E1 on rhBMP-2 immobilized titanium surface through polymer-mediated electrostatic interaction
Materials Science and Engineering: C Injectable hydrogels from enzyme-catalyzed crosslinking as BMSCs-laden scaffold for bone repair and regeneration
Biomaterials Science Development of CaCO 3 microsphere-based composite hydrogel for dual delivery of growth factor and Ca to enhance bone regeneration
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY The dose-time effects of fluoride on the expression and DNA methylation level of the promoter region of BMP-2 and BMP-7 in rats Pubmed: 32004919
Journal of Industrial and Engineering Chemistry Biofabrication and application of decellularized bone extracellular matrix for effective bone regeneration
BMC Musculoskeletal Disorders Synergistic Effect of Platelet-rich Fibrin Releasate and Bone Marrow Stem Cells in Preventing Bone Loss in Ovariectomized Mouse
Bioact Mater New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density 33842739
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