ELISA Kit for Factor Related Apoptosis (FAS)
CD95; ALPS1A; ALPS1-A; APO1; APT1; FAS1; FASTM; TNFRSF6; Fas Receptor; TNF Receptor Superfamily Member 6; Tumor Necrosis Factor Receptor Superfamily Member 6
- Product No.SEA030Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range39-2,500pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 15pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 448
For more details, please contact local distributors! US$ 2016
For more details, please contact local distributors! US$ 3808
For more details, please contact local distributors! US$ 31360
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Factor Related Apoptosis (FAS).
No significant cross-reactivity or interference between Factor Related Apoptosis (FAS) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Factor Related Apoptosis (FAS) and the recovery rates were calculated by comparing the measured value to the expected amount of Factor Related Apoptosis (FAS) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Factor Related Apoptosis (FAS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Factor Related Apoptosis (FAS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Factor Related Apoptosis (FAS) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|European Journal of Pharmacology||Montelukast abrogates rhabdomyolysis-induced acute renal failure via rectifying detrimental changes in antioxidant profile and systemic cytokines and apoptotic factors production ScienceDirect: S0014299912002580|
|J Sci Food Agric.||Beneficial effects of Allium sativum L. stem extract on lipid metabolism and antioxidant status in obese mice fed a high fat diet Wiley: Source|
|Tumor Biology||The diplotype Fas? 1377A/? 670G as a genetic marker to predict a lower risk of breast cancer in Chinese women Springer:Source|
|Naunyn Schmiedebergs Arch Pharmacol||The multi-kinase inhibitor pazopanib targets hepatic stellate cell activation and apoptosis alleviating progression of liver fibrosis PubMed: 26269412|
|Pharmacological Reports||The heat shock protein 90 inhibitor, 18-AAG, attenuates thioacetamide induced liver fibrosis in mice Science: Article|
|PLoS One||Aronia melanocarpa Extract Ameliorates Hepatic Lipid Metabolism through PPARγ2 Downregulation 10.1371|
|Molecular Diagnosis & Therapy||The Utility of Biomarkers in Osteoporosis Management pubmed:28271451|