Active Factor Related Apoptosis (FAS)

CD95; ALPS1A; ALPS1-A; APO1; APT1; FAS1; FASTM; TNFRSF6; Fas Receptor; TNF Receptor Superfamily Member 6; Tumor Necrosis Factor Receptor Superfamily Member 6

ACTIVITY TEST

FAS (Tumor necrosis factor receptor superfamily member 6) belongs to the tumor necrosis factor receptor superfamily. FAS contains a death domain, which has been shown to play a central role in the physiological regulation of programmed cell death. A binding ELISA assay was conducted to detect the association of FAS with TNFa. Briefly, FAS were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL FAS were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FAS pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of FAS and TNFa was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
European Journal of Pharmacology Montelukast abrogates rhabdomyolysis-induced acute renal failure via rectifying detrimental changes in antioxidant profile and systemic cytokines and apoptotic factors production ScienceDirect: S0014299912002580
J Sci Food Agric. Beneficial effects of Allium sativum L. stem extract on lipid metabolism and antioxidant status in obese mice fed a high fat diet Wiley: Source
Tumor Biology The diplotype Fas? 1377A/? 670G as a genetic marker to predict a lower risk of breast cancer in Chinese women Springer:Source
Naunyn Schmiedebergs Arch Pharmacol The multi-kinase inhibitor pazopanib targets hepatic stellate cell activation and apoptosis alleviating progression of liver fibrosis PubMed: 26269412
Pharmacological Reports The heat shock protein 90 inhibitor, 18-AAG, attenuates thioacetamide induced liver fibrosis in mice Science: Article
PLoS One Aronia melanocarpa Extract Ameliorates Hepatic Lipid Metabolism through PPARγ2 Downregulation 10.1371
Molecular Diagnosis & Therapy The Utility of Biomarkers in Osteoporosis Management pubmed:28271451
PLoS One Aronia melanocarpa Extract Ameliorates Hepatic Lipid Metabolism through PPARγ2 Downregulation pubmed:28081181
Journal of Microbiology, Immunology and Infection Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients pubmed:28655573
Journal of Functional Foods Polysaccharides from Cyclocarya paliurus: Chemical composition and lipid-lowering effect on rats challenged with high-fat diet 10.1016/j.jff.2017.07.020
Toxics Zinc Oxide Nanoparticles Induced Oxidative DNA Damage, Inflammation and Apoptosis in Rat's Brain after Oral Exposure Pubmed:29861430
Indian Journal of Medical and Paediatric Oncology The correlation between the level of doxorubicin-induced cardiac damage and serum soluble Fas in an experimental rat model Doi: 10.4103/ijmpo.ijmpo_82_17
Scientific Reports EGF-mediated reduced miR-92a-1-5p controls HTR-8/SVneo cell invasion through activation of MAPK8 and FAS which in turn increase MMP-2/-9 expression Pubmed: 32703964
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