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ELISA Kit for Insulin Like Growth Factor 1 (IGF1)

IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor

Specificity

This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor 1 (IGF1).
No significant cross-reactivity or interference between Insulin Like Growth Factor 1 (IGF1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Insulin Like Growth Factor 1 (IGF1) and the recovery rates were calculated by comparing the measured value to the expected amount of Insulin Like Growth Factor 1 (IGF1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-102 98
EDTA plasma(n=5) 81-92 86
heparin plasma(n=5) 95-104 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor 1 (IGF1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor 1 (IGF1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Insulin Like Growth Factor 1 (IGF1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-105% 84-95% 91-103% 84-103%
EDTA plasma(n=5) 90-103% 84-99% 82-104% 81-89%
heparin plasma(n=5) 81-88% 86-95% 79-101% 81-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Cellular & Molecular Biology Letters A microarray gene analysis of peripheral whole blood in normal adult male rats after long-term GH gene therapy PubMed: 20119855
Molecular Biology Reports Characterization of the specific and sustained GH1 expression induced by rAAV2/1 in normal adult male rats PubMed: 20204528
Regulatory Peptides The vagus nerve as a regulator of growth hormone secretion PubMed: 21056594
Vascular Cell Insulin-Like Growth Factor-1 (IGF-1) Reduces Ischemic Changes and Increases Circulating Angiogenic Factors in Experimentally - Induced Myocardial Infarction in Rats PubMed: 21651821
American Journal of Respiratory and Critical Care Medicine Physical activity attenuates intermittent hypoxia-induced spatial learning deficits and oxidative stress. PubMed: 20224062
Journal of Pediatric Surgery The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass model PubMed: 20438940
The Veterinary Journal Serum insulin-like growth factor type 1 concentrations in healthy dogs and dogs with spontaneous primary hypothyroidism PubMed: 21546289
Developmental Biology Effects of monochromatic light on developmental changes in satellite cell population of pectoral muscle in broilers during early posthatch period Wiley: source
Journal of Neuroendocrinology Interleukin-6 Regulates the Expression of Hypothalamic Neuropeptides Involved in Body Weight in a Gender-Dependent Way Wiley: source
International Journal of Molecular Sciences Insulin Like Growth Factor-1 (IGF-1) Causes Overproduction of IL-8, an Angiogenic Cytokine and Stimulates Neovascularization in Isoproterenol-Induced Myocardial Infarction in Rats Mdpi: 8562
Pharmacology Protective Effect of Amlodipine on Rat Bone Tissue after Orchidectomy Karger: 335491
Bulletin of the Veterinary Institute in Pulawy Maternal Glucocorticoid Treatment as a Model for Examining Foetal Gender-Specific Effects on the Bone Metabolism of Neonatal Piglets Degruyter: Source
BMC cell biology BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopause PubMed: PMC3640998
Journal of Veterinary Science Evaluation of IGF-I levels and serum protein profiles of diabetic cats and dogs PubMed: PMC3232391
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source
Pesquisa Veterinária Brasileira Porcine follicular fluid concentration of free insulin-like growth factor-I collected from different diameter ovarian follicles Scielo: Source
international Journal of food and nutritional sciences EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI Ijfans: Source
Theriogenology Role of insulin-like growth factor-I and follicular fluid from ovarian follicles with different diameters on porcine oocyte maturation and fertilization in vitro ScienceDirect: S0093691X13001659
Biosensors and Bioelectronics Electrochemical immunosensor for the determination of insulin-like growth factor-1 using electrodes modified with carbon nanotubes–poly(pyrrole propionic acid) hybrids ScienceDirect: S0956566313005629
Electromagnetic Biology and Medicine The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar rats Informahealthcare: Source
Nutrition and Cancer A Pilot Study Comparing the Effect of Flaxseed, Aromatase Inhibitor, and the Combination on Breast Tumor Biomarkers Tandfonline: Source
Bases de Dados da Pesquisa Agropecuária CONCENTRACOES SéRICAS DE INSULINA NO PóS-PARTO RECENTE DE VACAS DE CORTE DE DIFERENTES GENóTIPOS CRIADAS NO SUL DO BRASIL Embrapa: Source
International Journal of Endocrinology Negative Energy Balance Induced by Paradoxical Sleep Deprivation Causes Multicompartmental Changes in Adipose Tissue and Skeletal Muscle Cepebr:Source
J Neurol Sci. The effect of topiramate and lamotrigine on rat bone mass, structure and metabolism. Pubmed:24629477
Reproductive Sciences Effects of Resveratrol on Ovarian Morphology, Plasma Anti-Mullerian Hormone, IGF-1 Levels, and Oxidative Stress Parameters in a Rat Model of Polycystic Ovary Syndrome Pubmed:25667201
Int J Clin Exp Med Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes PubMed: 26379831
Int J Clin Exp Med. The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell PubMed: 26628972
Animal and Biomedical Sciences The use of herb mix and lucerne to increase growth rates of dairy heifers System: Files
Neonatal Biology Maternal Nutrition with Β-Hydroxy-Β-Methylbutyrate as Strong Determinants of the Development of Newborn Offspring in Pigs Journals: 1000183
Dig Dis Sci The Protective Effects of Exclusive Enteral Nutrition Formulas on Growth Factor Expression and the Proximal Tibial Epiphyseal Growth Plate in a TNBS-Induced IBD Rat Model PubMed: 25708898
Tumor Biology Hypoxia-induced secretion of IL-12 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma PubMed: 26695151
Forage Res RELATIONSHIP OF RESIDUAL FEED INTAKE WITH BLOOD METABOLITES AND HORMONES IN SAHIWAL FEMALE CALVES Wp-Content: Uploads
S Caracteriza Teses: Disponiveis
Photochemistry AND Photobiology Melatonin Mediates Monochromatic Light‐induced Insulin‐like Growth Factor 1 Secretion of Chick Liver: Involvement of Membrane Receptors Pubmed:27128575
Pediatric Pulmonology Protective effect of vitamin D against hyperoxia‐induced lung injury in newborn rats Pubmed:27291304
Hippocampus A single bout of resistance exercise improves memory consolidation and increases the expression of synaptic proteins in the hippocampus Pubmed:27008926
Neurotoxicity Research Cigarette Smoke Delays Regeneration of the Olfactory Epithelium in Mice Pubmed:27003941
ANNALS OF ANIMAL SCIENCE Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens journalprintahead:87
Annals of Animal Science Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens view:87
African Health Sciences Effects of acute exercise on salivary free insulin-like growth factor 1 and interleukin 10 in sportsmen. pubmed:27605972
PLoS One. Paradoxical Sleep Deprivation Causes Cardiac Dysfunction and the Impairment Is Attenuated by Resistance Training. pubmed:27880816
Biochemistry and Cell Biology Chronic intermittent hypoxia disturbs insulin secretion and causes pancreatic injury via theMAPK signaling pathway. pubmed:28177762
Neurochemical Research Resistance Exercise Reduces Seizure Occurrence, Attenuates Memory Deficits and Restores BDNF Signaling in Rats with Chronic Epilepsy article:10.1007
Journal of Agricultural Biotechnology GH蛋白诱导鸡原代成肌细胞表达IGF1条件优化 10697
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