ELISA Kit for Insulin Like Growth Factor 1 (IGF1)
IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor
- Product No.SEA050Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.069ng/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 438
For more details, please contact local distributors! US$ 1971
For more details, please contact local distributors! US$ 3723
For more details, please contact local distributors! US$ 30660
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor 1 (IGF1).
No significant cross-reactivity or interference between Insulin Like Growth Factor 1 (IGF1) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Insulin Like Growth Factor 1 (IGF1) and the recovery rates were calculated by comparing the measured value to the expected amount of Insulin Like Growth Factor 1 (IGF1) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor 1 (IGF1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor 1 (IGF1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Insulin Like Growth Factor 1 (IGF1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Cellular & Molecular Biology Letters||A microarray gene analysis of peripheral whole blood in normal adult male rats after long-term GH gene therapy PubMed: 20119855|
|Molecular Biology Reports||Characterization of the specific and sustained GH1 expression induced by rAAV2/1 in normal adult male rats PubMed: 20204528|
|Regulatory Peptides||The vagus nerve as a regulator of growth hormone secretion PubMed: 21056594|
|Vascular Cell||Insulin-Like Growth Factor-1 (IGF-1) Reduces Ischemic Changes and Increases Circulating Angiogenic Factors in Experimentally - Induced Myocardial Infarction in Rats PubMed: 21651821|
|American Journal of Respiratory and Critical Care Medicine||Physical activity attenuates intermittent hypoxia-induced spatial learning deficits and oxidative stress. PubMed: 20224062|
|Journal of Pediatric Surgery||The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass model PubMed: 20438940|
|The Veterinary Journal||Serum insulin-like growth factor type 1 concentrations in healthy dogs and dogs with spontaneous primary hypothyroidism PubMed: 21546289|
|Developmental Biology||Effects of monochromatic light on developmental changes in satellite cell population of pectoral muscle in broilers during early posthatch period Wiley: source|
|Journal of Neuroendocrinology||Interleukin-6 Regulates the Expression of Hypothalamic Neuropeptides Involved in Body Weight in a Gender-Dependent Way Wiley: source|
|International Journal of Molecular Sciences||Insulin Like Growth Factor-1 (IGF-1) Causes Overproduction of IL-8, an Angiogenic Cytokine and Stimulates Neovascularization in Isoproterenol-Induced Myocardial Infarction in Rats Mdpi: 8562|
|Pharmacology||Protective Effect of Amlodipine on Rat Bone Tissue after Orchidectomy Karger: 335491|
|Bulletin of the Veterinary Institute in Pulawy||Maternal Glucocorticoid Treatment as a Model for Examining Foetal Gender-Specific Effects on the Bone Metabolism of Neonatal Piglets Degruyter: Source|
|BMC cell biology||BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopause PubMed: PMC3640998|
|Journal of Veterinary Science||Evaluation of IGF-I levels and serum protein profiles of diabetic cats and dogs PubMed: PMC3232391|
|PLoS ONE||A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source|
|Pesquisa Veterinária Brasileira||Porcine follicular fluid concentration of free insulin-like growth factor-I collected from different diameter ovarian follicles Scielo: Source|
|international Journal of food and nutritional sciences||EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI Ijfans: Source|
|Theriogenology||Role of insulin-like growth factor-I and follicular fluid from ovarian follicles with different diameters on porcine oocyte maturation and fertilization in vitro ScienceDirect: S0093691X13001659|
|Biosensors and Bioelectronics||Electrochemical immunosensor for the determination of insulin-like growth factor-1 using electrodes modified with carbon nanotubes–poly(pyrrole propionic acid) hybrids ScienceDirect: S0956566313005629|
|Electromagnetic Biology and Medicine||The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar rats Informahealthcare: Source|
|Nutrition and Cancer||A Pilot Study Comparing the Effect of Flaxseed, Aromatase Inhibitor, and the Combination on Breast Tumor Biomarkers Tandfonline: Source|
|Bases de Dados da Pesquisa Agropecuária||CONCENTRACOES SéRICAS DE INSULINA NO PóS-PARTO RECENTE DE VACAS DE CORTE DE DIFERENTES GENóTIPOS CRIADAS NO SUL DO BRASIL Embrapa: Source|
|International Journal of Endocrinology||Negative Energy Balance Induced by Paradoxical Sleep Deprivation Causes Multicompartmental Changes in Adipose Tissue and Skeletal Muscle Cepebr:Source|
|J Neurol Sci.||The effect of topiramate and lamotrigine on rat bone mass, structure and metabolism. Pubmed:24629477|
|Reproductive Sciences||Effects of Resveratrol on Ovarian Morphology, Plasma Anti-Mullerian Hormone, IGF-1 Levels, and Oxidative Stress Parameters in a Rat Model of Polycystic Ovary Syndrome Pubmed:25667201|
|Int J Clin Exp Med||Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes PubMed: 26379831|
|Int J Clin Exp Med.||The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell PubMed: 26628972|
|Animal and Biomedical Sciences||The use of herb mix and lucerne to increase growth rates of dairy heifers System: Files|
|Neonatal Biology||Maternal Nutrition with Β-Hydroxy-Β-Methylbutyrate as Strong Determinants of the Development of Newborn Offspring in Pigs Journals: 1000183|
|Dig Dis Sci||The Protective Effects of Exclusive Enteral Nutrition Formulas on Growth Factor Expression and the Proximal Tibial Epiphyseal Growth Plate in a TNBS-Induced IBD Rat Model PubMed: 25708898|
|Tumor Biology||Hypoxia-induced secretion of IL-12 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma PubMed: 26695151|
|Forage Res||RELATIONSHIP OF RESIDUAL FEED INTAKE WITH BLOOD METABOLITES AND HORMONES IN SAHIWAL FEMALE CALVES Wp-Content: Uploads|
|S||Caracteriza Teses: Disponiveis|
|Photochemistry AND Photobiology||Melatonin Mediates Monochromatic Light‐induced Insulin‐like Growth Factor 1 Secretion of Chick Liver: Involvement of Membrane Receptors Pubmed:27128575|
|Pediatric Pulmonology||Protective effect of vitamin D against hyperoxia‐induced lung injury in newborn rats Pubmed:27291304|
|Hippocampus||A single bout of resistance exercise improves memory consolidation and increases the expression of synaptic proteins in the hippocampus Pubmed:27008926|
|Neurotoxicity Research||Cigarette Smoke Delays Regeneration of the Olfactory Epithelium in Mice Pubmed:27003941|
|ANNALS OF ANIMAL SCIENCE||Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens journalprintahead:87|
|Annals of Animal Science||Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens view:87|
|African Health Sciences||Effects of acute exercise on salivary free insulin-like growth factor 1 and interleukin 10 in sportsmen. pubmed:27605972|
|PLoS One.||Paradoxical Sleep Deprivation Causes Cardiac Dysfunction and the Impairment Is Attenuated by Resistance Training. pubmed:27880816|
|Biochemistry and Cell Biology||Chronic intermittent hypoxia disturbs insulin secretion and causes pancreatic injury via theMAPK signaling pathway. pubmed:28177762|
|Neurochemical Research||Resistance Exercise Reduces Seizure Occurrence, Attenuates Memory Deficits and Restores BDNF Signaling in Rats with Chronic Epilepsy article:10.1007|
|Journal of Agricultural Biotechnology||GH蛋白诱导鸡原代成肌细胞表达IGF1条件优化 10697|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
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|KSA050Ra01||ELISA Kit DIY Materials for Insulin Like Growth Factor 1 (IGF1)||Main materials for "Do It (ELISA Kit) Yourself".|