ELISA Kit for Interleukin 13 (IL13)

ALRH; BHR1; P600; IL-13; NC30

Specificity

This assay has high sensitivity and excellent specificity for detection of Interleukin 13 (IL13).
No significant cross-reactivity or interference between Interleukin 13 (IL13) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 13 (IL13) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 13 (IL13) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 91-98 95
EDTA plasma(n=5) 83-103 98
heparin plasma(n=5) 80-89 84

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 13 (IL13) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 13 (IL13) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 13 (IL13) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-102% 78-91% 78-93% 89-97%
EDTA plasma(n=5) 99-105% 90-104% 82-104% 92-105%
heparin plasma(n=5) 84-104% 93-105% 92-101% 96-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Critical Care Medicine Systemic release of cytokines and heat shock proteins in porcine models of polytrauma and hemorrhage PubMed: PMC3383056
Int J Clin Exp Pathol Downregulation of SUMF2 gene in ovalbumin-induced rat model of allergic inflammation PubMed: 26722390
Advances in Experimental Medicine and Biology  Antiinflammatory Effect of N-Acetylcysteine Combined with Exogenous Surfactant in Meconium-Induced Lung Injury Pubmed:27283193
Molecular Immunology Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by reCavia (Guinea pig )lating caveolin-1 through TLR2/MyD88/NF-ŚĘB in murine model of asthma Pubmed:27289030
Journal of Agricultural Biotechnology Gln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells 10610
BMC Veterinary Research Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis pubmed:27553600
Molecular Immunology Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by regulating caveolin-1 through TLR2/MyD88/NF-κB in murine model of asthma pubmed:27289030
Physiological Research Lung inflammatory and oxidative alterations after exogenous surfactant therapy fortified with budesonide in rabbit model of meconium aspiration syndrome. pubmed:28006947
Gut Pathog. Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice pubmed:28250847
Veterinary Immunology and Immunopathology Cytokine levels in colostrum and in foals' serum pre- and post-suckling pubmed:28242000
Asian Pacific Society of Respirology Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice pubmed:28122397
Journal of Autoimmunity Commensal bacteria aggravate allergic asthma via NLRP3/IL-1β signaling in post-weaning mice 10.1016:j.jaut.2018.07.003
bulletin of experimental biology and medicine Quantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal Origin Pubmed: 31183654
life sciences Melatonin biosynthesis restored by CpG oligodeoxynucleotides attenuates allergic airway inflammation via regulating NLRP3 inflammasome Pubmed: 31738882
Frontiers in Immunology TLR2-Melatonin Feedback Loop Regulates the Activation of NLRP3 Inflammasome in Murine Allergic Airway Inflammation Pubmed: 32117301
Parasites & Vectors Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism … Pubmed: 32680553
Biomedicine & Pharmacotherapy Qingfei oral liquid alleviates airway hyperresponsiveness and mucus hypersecretion via TRPV1 signaling in RSV-infected asthmatic mice Pubmed: 32521453
Journal of Traditional Chinese Medical Sciences Mahuang Xixin Fuzi Decoction protects against ovalbumin-induced allergic rhinitis by inhibiting type 2 innate lymphoid cells in mice
Traditional Medicine Research Qingfei oral liquid downregulates TRPV1 expression to reduce airway inflammation and mucus hypersecretion injury caused by respiratory syncytial virus infection …
Applied materials today Tanshinone IIA-loaded aligned microfibers facilitate stem cell recruitment and capillary formation by inducing M2 macrophage polarization
Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota
Biomed Pharmacother Qingfei oral liquid inhibited autophagy to alleviate inflammation via mTOR signaling pathway in RSV-infected asthmatic mice 33706133
BIOMATERIALS Aligned microfiber-induced macrophage polarization to guide schwann-cell-enabled peripheral nerve regeneration 33813259
PLoS One Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies 33999928
Anti-inflammatory activity of non-selective PDE inhibitor aminophylline on the lung tissue and respiratory parameters in animal model of ARDS
Catalog No. Related products for research use of Chicken (Gallus) Organism species Applications (RESEARCH USE ONLY!)
RPA060Ga01 Recombinant Interleukin 13 (IL13) Positive Control; Immunogen; SDS-PAGE; WB.
PAA060Ga01 Polyclonal Antibody to Interleukin 13 (IL13) WB; IHC; ICC; IP.
MAA060Ga21 Monoclonal Antibody to Interleukin 13 (IL13) WB; IHC; ICC; IP.
SEA060Ga ELISA Kit for Interleukin 13 (IL13) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA060Ga Multiplex Assay Kit for Interleukin 13 (IL13) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.