ELISA Kit for Tumor Necrosis Factor Beta (TNFb)

LT; LTA; TNF-B; TNFSF1; Tumor Necrosis Factor Ligand Superfamily Member 1; Lymphotoxin Alpha

Specificity

This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Beta (TNFb).
No significant cross-reactivity or interference between Tumor Necrosis Factor Beta (TNFb) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Tumor Necrosis Factor Beta (TNFb) and the recovery rates were calculated by comparing the measured value to the expected amount of Tumor Necrosis Factor Beta (TNFb) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-89 81
EDTA plasma(n=5) 95-103 98
heparin plasma(n=5) 96-104 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Beta (TNFb) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Beta (TNFb) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tumor Necrosis Factor Beta (TNFb) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-105% 86-94% 99-105% 91-105%
EDTA plasma(n=5) 84-99% 96-105% 94-105% 80-103%
heparin plasma(n=5) 98-105% 91-99% 99-105% 79-89%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Applied Physiology Simvastatin attenuates sympathetic hyperinnervation to prevent atrial fibrillation during the postmyocardial infarction remodeling process PubMed: 22984252
Journal of Translational Medicine Effectiveness of pure argon for renal transplant preservation in a preclinical pig model of heterotopic autotransplantation Pubmed:26847569
International Journal of Stem Cell Research and Transplantation Protective Role of Bone Marrow Derived Mesenchymal Stem Cells-Conditioned Medium in the Infarcted Myocardium: The Potential Role of Selected Cytokines IJST-2328-3548-04-803
Probiotics Antimicrob Proteins Effect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a … Pubmed:29305686
Parasites & Vectors Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism … Pubmed: 32680553
Microbiome changes in patients with chronic heart failure with preserved ejection fraction correlate with fibrosis markers: Description of a Russian cohort
BMC MUSCULOSKELETAL DISORDERS Elevated lymphotoxin-¦Á (TNF¦Â) is associated with intervertebral disc degeneration 33441130
Catalog No. Related products for research use of Sus scrofa; Porcine (Pig) Organism species Applications (RESEARCH USE ONLY!)
RPA134Po01 Recombinant Tumor Necrosis Factor Beta (TNFb) Positive Control; Immunogen; SDS-PAGE; WB.
APA134Po01 Active Tumor Necrosis Factor Beta (TNFb) Cell culture; Activity Assays.
PAA134Po01 Polyclonal Antibody to Tumor Necrosis Factor Beta (TNFb) WB; IHC; ICC; IP.
MAA134Po21 Monoclonal Antibody to Tumor Necrosis Factor Beta (TNFb) WB; IHC; ICC; IP.
SEA134Po ELISA Kit for Tumor Necrosis Factor Beta (TNFb) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA134Po Multiplex Assay Kit for Tumor Necrosis Factor Beta (TNFb) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.