ELISA Kit for Collagen Type III (COL3)

Specificity

This assay has high sensitivity and excellent specificity for detection of Collagen Type III (COL3).
No significant cross-reactivity or interference between Collagen Type III (COL3) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Collagen Type III (COL3) and the recovery rates were calculated by comparing the measured value to the expected amount of Collagen Type III (COL3) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-102 88
EDTA plasma(n=5) 81-93 87
heparin plasma(n=5) 85-99 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type III (COL3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type III (COL3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Collagen Type III (COL3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 96-103% 87-101% 81-99% 91-101%
EDTA plasma(n=5) 78-90% 91-98% 81-94% 78-88%
heparin plasma(n=5) 89-99% 94-105% 79-92% 78-88%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
nephrology dialysis transplantation Triptolide inhibits extracellular matrix protein synthesis by suppressing the Smad2 but not the MAPK pathway in TGF-β1-stimulated NRK-49F cells OxfordJournals: 3180
Acta Otolaryngol Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa PubMed: 19958244
Bulletin of Experimental Biology and Medicine Modification of collagen formation by mesenchymal stem cells isolated from human adipose tissue in culture and after autotransplantation for abdominal hernia plasty Pubmed: 24319714
Age( dordr). In vitro tenocyte metabolism in aging and oestrogen deficiency Springer: Source
Stem Cell Research Therapy Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly Pubmed: 24739658
International Journal of Advanced Research Effect of nandrolone and/or whey protein on the soleus muscle and testis of adult male albino rats Com:Source
PLoS One. Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media Pubmed:Pmc4130592
Connective Tissue Research Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes Pubmed:25333747
Laryngoscope. Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria Pubmed:25545625
Cardiology KLF15 is an essential negative regulatory factor for the cardiac remodeling response to pressure overload Pubmed:25633973
physiological research The skin remodeling in type 1 diabetes and insulin resistance animal models PubMed: 26047379
Physiol Res The skin remodeling in type 1 diabetes and insulin resistance animal models. PubMed: 26047379
Archives of Plastic Surgery Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile Hemangiomas PubMed: 26430624
Life Sci Pituitary tumor transforming gene as a novel regulatory factor of liver fibrosis PubMed: 25936962
Molecular and Cellular Biochemistry SVVYGLR motif of the thrombin-cleaved N-terminal osteopontin fragment enhances the synthesis of collagen type III in myocardial fibrosis PubMed: 26112906
Cardiology KLF16 Is an Essential Negative Regulatory Factor for the Cardiac Remodeling Response to Pressure Overload PubMed: 25633973
Int J Clin Exp Med Uighur medicine abnormal savda munzip (ASMq) suppresses expression of collagen and TGF-β1 with concomitant induce Smad7 in human hypertrophic scar … PubMed: 26309506
scientific reports IL-17A mediates inflammatory and tissue remodelling events in early human tendinopathy Pubmed:27263531
Кардіологія Інфаркт міокарда, ускладнений аневризмою лівого шлуночка: клінічні і структурно-функціональні особливості перебігу, діагностичні, терапевтичні та прогностичні аспекти bitstream:123456789
International Journal of Molecular Sciences Plasma LncRNA-ATB, a Potential Biomarker for Diagnosis of Patients with Coal Workers'Pneumoconiosis: A Case-Control Study. pubmed:27556453
BMC Cancer Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent andmitochondrial pathways in mammalian hepatic stellate cells. pubmed:27461108
Sci Rep. Genomic reprograming analysis of the Mesothelial to Mesenchymal Transition identifies biomarkers in peritoneal dialysis patients. pubmed:28327551
The American Journal of Chinese Medicine Saikosaponin a Induces Apoptosis through Mitochondria-Dependent Pathway in Hepatic Stellate Cells pubmed:28231747
Biomedical and Environmental Sciences Crystalline Silica Promotes Rat Fibrocyte Differentiation in Vitro, and Fibrocytes Participate in Silicosis in Vivo pubmed:29081339
Oxidative Medicine and Cellular Longevity Effects of the (Pro) renin Receptor on Cardiac Remodeling and Function in a Rat Alcoholic Cardiomyopathy Model via the PRR-ERK1/2-NOX4 Pathway
Cytokine Effect of proinflammatory cytokines on endometrial collagen and metallopeptidase expression during the course of equine endometrosis Pubmed: 31265984
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Pantoea agglomerans chronic exposure induces epithelial-mesenchymal transition in human lung epithelial cells and mice lungs Pubmed: 32146192
in vitro cellular & developmental biology-animal Distinct differences in hypoxic responses between human oral mucosa and skin fibroblasts in a 3D collagen matrix Pubmed: 32588253
ACS Biomaterials Science & Engineering Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction
Stem Cell Research & Therapy Enhanced cell survival and therapeutic benefits of IL-10-expressing multipotent mesenchymal stromal cells for muscular dystrophy 33541428
journal of biomedical materials research part B Cell sheet technology: Influence of culture conditions on in vitro©\cultivated corneal stromal tissue for regenerative therapies of the ocular surface 33538123
PLoS One Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies 33999928
BMC MOLECULAR BIOLOGY Uncarboxylated osteocalcin alleviates the inhibitory effect of high glucose on osteogenic differentiation of mouse bone marrow¨Cderived mesenchymal stem?¡­ 33906607
Russian Journal of Cardiology Early diagnosis of myocardial fibrosis in patients with epicardial obesity
Manuscript title Microparticles Containing MiR-625-5p In Coronary Artery Reduce Myocardial Fibrosis After Myocardial Infarction
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
NPA176Ra01 Native Collagen Type III (COL3) Positive Control; Immunogen; SDS-PAGE; WB.
SEA176Ra ELISA Kit for Collagen Type III (COL3) Enzyme-linked immunosorbent assay for Antigen Detection.
WEA176Ra Wide-range ELISA Kit for Collagen Type III (COL3) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA176Ra Multiplex Assay Kit for Collagen Type III (COL3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.