ELISA Kit for Collagen Type III (COL3)
- Product No.SEA176Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.312-20ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.122ng/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Collagen Type III (COL3).
No significant cross-reactivity or interference between Collagen Type III (COL3) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Collagen Type III (COL3) and the recovery rates were calculated by comparing the measured value to the expected amount of Collagen Type III (COL3) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type III (COL3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type III (COL3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Collagen Type III (COL3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|nephrology dialysis transplantation||Triptolide inhibits extracellular matrix protein synthesis by suppressing the Smad2 but not the MAPK pathway in TGF-β1-stimulated NRK-49F cells OxfordJournals: 3180|
|Acta Otolaryngol||Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa PubMed: 19958244|
|Bulletin of Experimental Biology and Medicine||Modification of collagen formation by mesenchymal stem cells isolated from human adipose tissue in culture and after autotransplantation for abdominal hernia plasty Pubmed: 24319714|
|Age( dordr).||In vitro tenocyte metabolism in aging and oestrogen deficiency Springer: Source|
|Stem Cell Research Therapy||Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly Pubmed: 24739658|
|International Journal of Advanced Research||Effect of nandrolone and/or whey protein on the soleus muscle and testis of adult male albino rats Com:Source|
|PLoS One.||Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media Pubmed:Pmc4130592|
|Connective Tissue Research||Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes Pubmed:25333747|
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|Archives of Plastic Surgery||Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile Hemangiomas PubMed: 26430624|
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|BMC Cancer||Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent andmitochondrial pathways in mammalian hepatic stellate cells. pubmed:27461108|
|Sci Rep.||Genomic reprograming analysis of the Mesothelial to Mesenchymal Transition identifies biomarkers in peritoneal dialysis patients. pubmed:28327551|
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|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
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