• Promotion
ELISA Kit for Galectin 1 (GAL1)

LGALS1; GBP; HLBP14; Galaptin; Lectin,Galactoside-Binding Soluble 1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; Putative MAPK-activating protein PM12; S-Lac lectin

Specificity

This assay has high sensitivity and excellent specificity for detection of Galectin 1 (GAL1).
No significant cross-reactivity or interference between Galectin 1 (GAL1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Galectin 1 (GAL1) and the recovery rates were calculated by comparing the measured value to the expected amount of Galectin 1 (GAL1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-102 95
EDTA plasma(n=5) 91-98 95
heparin plasma(n=5) 84-96 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galectin 1 (GAL1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galectin 1 (GAL1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Galectin 1 (GAL1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-93% 79-89% 92-104% 78-96%
EDTA plasma(n=5) 88-104% 94-101% 99-105% 80-96%
heparin plasma(n=5) 89-101% 90-104% 97-105% 94-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Reference
International Journal of Oncology Galectin-1-mediated cell adhesion, invasion and cell death in human anaplastic large cell lymphoma: Regulatory roles of cell surface glycans Pubmed:24589677
Clinica Chimica Acta Galectin-1 and galectin-3: plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population Pubmed:25578395
Journal of Perinatal Medicine Cord blood galectin-1 and-3 concentrations in term pregnancies with normal restricted and increased fetal growth PubMed: 24717333
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA321Ra01 Recombinant Galectin 1 (GAL1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA321Ra02 Recombinant Galectin 1 (GAL1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA321Ra01 Polyclonal Antibody to Galectin 1 (GAL1) WB; IHC; ICC; IP.
PAA321Ra02 Polyclonal Antibody to Galectin 1 (GAL1) WB; IHC; ICC; IP.
LAA321Ra81 FITC-Linked Polyclonal Antibody to Galectin 1 (GAL1) WB; IHC; ICC; IF.
LAA321Ra71 Biotin-Linked Polyclonal Antibody to Galectin 1 (GAL1) WB; IHC; ICC.
MAA321Ra22 Monoclonal Antibody to Galectin 1 (GAL1) WB; IHC; ICC; IP.
SEA321Ra ELISA Kit for Galectin 1 (GAL1) Enzyme-linked immunosorbent assay for Antigen Detection.
KSA321Ra01 ELISA Kit DIY Materials for Galectin 1 (GAL1) Main materials for "Do It (ELISA Kit) Yourself".