ELISA Kit for Osteocalcin (OC)

BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein

Specificity

This assay has high sensitivity and excellent specificity for detection of Osteocalcin (OC).
No significant cross-reactivity or interference between Osteocalcin (OC) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Osteocalcin (OC) and the recovery rates were calculated by comparing the measured value to the expected amount of Osteocalcin (OC) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-98 94
EDTA plasma(n=5) 90-98 93
heparin plasma(n=5) 80-90 82

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Osteocalcin (OC) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osteocalcin (OC) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Osteocalcin (OC) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-93% 92-99% 84-98% 94-105%
EDTA plasma(n=5) 79-97% 85-95% 80-97% 80-102%
heparin plasma(n=5) 80-103% 92-101% 79-88% 79-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Acta Biomaterialia Surface engineering of titanium with potassium hydroxide and its effects on the growth behavior of mesenchymal stem cells PubMed: 19963080
Annals of RSCB Biochemical study of the influence of ad type biophytomodulators in healing of bone defects in rats. Analelesnbc: source
Asian-Australasian Association of Animal Societies Variability of Osteocalcin Status in Chinese Holstein Cattle: Do Phylogeny, Vitamin D or Gene Polymorphisms Matter? Ajas: source
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J Anim Physiol Anim Nutr (Berl). Morphological changes of the cartilage and bone in newborn piglets evoked by experimentally induced glucocorticoid excess during pregnancy Pubmed: 22716040
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Nutr Hosp. Effect of the “protein diet” and bone tissue. Pubmed: 24483972
International Journal of Cardiology Valvular osteoclasts in calcification and aortic valve stenosis severity. Pubmed: 23452891
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Food Sci Nutr. Effects and mechanisms of 8‐prenylnaringenin on osteoblast MC3T3‐E1 and osteoclast‐like cells RAW264. 7 Pubmed:Pmc4221832
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FEBS Letters Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells Pubmed:25542352
American Diabetes Association Pathophysiological mechanism of bone loss in type 2 diabetes involves inverse regulation of osteoblast function by PPARγ coactivator-1α and skeletal muscle atrogenes: adiponectin receptor 1 as a potential target for reversing diabetes-induced osteopenia Pubmed:25633418
International Journal of Nanomedicine Immobilizing osteogenic growth peptide with and without fibronectin on a titanium surface: effects of loading methods on mesenchymal stem cell differentiation Pubmed:25678785
Medical Journal fo the Islamic Republic of Iran Transplantation of mesenchymal stem cells, recombinant human BMP-2,and their combination in accelerating the union after osteotomy and increasing, the mechanical strength of extracorporeally irradiated femoral autograft in rat models Pubmed:25679008
BioMed Research International Inhibition of osteoclast activation by phloretin through disturbing αvβ3 integrin-c-Src pathway Pubmed:25834823
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journal of hard Tissue Biology Biological Evaluation of a Prototype Material made of Polyglycolic Acid and Hydroxyapatite Article: Jhtb
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Journal of Alloys and Compounds Enhanced biocompatible and hemocompatible nano/micro porous surface as a biological scaffold for functionalizational and biointegrated implants science:S0045653516302016
Bone The effects of strength training and raloxifene on bone health in aging ovariectomized rats Pubmed:26812611
Biological Trace Element Research Effect of Sodium Fluoride on Bone Biomechanical and Histomorphometric Parameters and on Insulin Signalingand Insulin Sensitivity in Ovariectomized Rats. Pubmed:26876375
ANNALS OF ANIMAL SCIENCE Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens journalprintahead:87
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Protective Effect of Soybean and Mushroom against Glucocorticoid-Induced Osteoporosis in Female Rats :
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international journal of molecular sciences Evidence of the Role of R-Spondin 1 and Its Receptor Lgr4 in the Transmission of Mechanical Stimuli to Biological Signals for Bone Formation. pubmed:28272338
Applied Surface Science The controlled release of simvastatin from TiO2 nanotubes to promote osteoblast differentiation and inhibit osteoclast resorption S0169433216326861
Nutrition. Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats. pubmed:27908549
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Biomedicine & Pharmacotherapy Fast and long acting neoflavonoids dalbergin isolated from Dalbergia sissoo heartwood is osteoprotective in ovariectomized model of osteoporosis: Osteoprotective … S0753332216308484
Experimental and Therapeutic Medicine Serum N-terminal telopeptide of type I collagen as an early marker of fracture nonunion in rabbits. pubmed:28105092
Institute of Pharmacology Effects of long-term administration of pantoprazole on bone mineral density in young male rats. pubmed:27552060
Indian Journal of Orthopaedics N-terminal telopeptides of type I collagen and bone mineral density for early diagnosis ofnonunion: An experimental study in rabbits. pubmed:27512225
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Mater Sci Eng C Mater Biol Appl. Synthesis of polypyrrole nanowires with positive effect on MC3T3-E1 cell functions through electrical stimulation. pubmed:27987727
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