Polyclonal Antibody to Osteocalcin (OC)

BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against OC. It has been selected for its ability to recognize OC in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
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Asian-Australasian Association of Animal Societies Variability of Osteocalcin Status in Chinese Holstein Cattle: Do Phylogeny, Vitamin D or Gene Polymorphisms Matter? Ajas: source
Nutrition Dyslipidemic high-fat diet affects adversely bone metabolism in mice associated with impaired antioxidant capacity ScienceDirect: S0899900709004717
Experimental Biology and Medicine Osteogenic activity of silymarin through enhancement of alkaline phosphatase and osteocalcin in osteoblasts and tibia-fractured mice Rsm: source
Archives of Oral Biology Oxytocin promotes bone formation during the alveolar healing process in old acyclic female rats ScienceDirect: S0003996912001021
22 Regulation of the differentiation of mesenchymal stem cells in vitro and osteogenesis in vivo by microenvironmental modification of titanium alloy surfaces ScienceDirect: S0142961212000865
Life Sciences Exendin-4 Increases Bone Mineral Density in Type 2 Diabetic OLETF Rats Potentially Through the Down-Regulation of SOST/Sclerostin in Osteocytes PubMed: 23357248
Romanian Journal of Morphology and Embryology Serum changes induced by intramedullar experimental administration of bisphosphonates PubMed: 21424089
International Journal of Cardiology Valvular osteoclasts in calcification and aortic valve stenosis severity PubMed: 23452891
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Journal of Applied Toxicology Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in mice Wiley: Source
Chinese Medicine Puerarin promotes osteogenesis and inhibits adipogenesis in vitro PubMed: PMC3765709
Nephrology Dialysis Transplantation Magnesium prevents phosphate-induced calcification in human aortic vascular smooth muscle cells PubMed: PMC3611891
J Anim Physiol Anim Nutr (Berl). Morphological changes of the cartilage and bone in newborn piglets evoked by experimentally induced glucocorticoid excess during pregnancy Pubmed: 22716040
Journal of Cranio-Maxillofacial Surgery The use of Tri-Calcium Phosphate (TCP) and stem cells for the regeneration of osteoperiosteal critical-size mandibular bony defects, an in vitro and preclinical study Pubmed: 24485270
Nutr Hosp. Effect of the “protein diet” and bone tissue. Pubmed: 24483972
International Journal of Cardiology Valvular osteoclasts in calcification and aortic valve stenosis severity. Pubmed: 23452891
Food Science & Nutrition Effects and mechanisms of 8-prenylnaringenin on osteoblast MC3T3-E1 and osteoclast-like cells RAW264.7 Onlinelibrary: fsn3.109
Nutricion Hospitalaria Effect of the “protein diet” and bone tissue Aulamedica: Source
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Basic & Clinical Pharmacology & Toxicology Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory Conditions Wiley:Source
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Journal of Cranio-Maxillofacial Surgery The use of TriCalcium Phosphate (TCP) and stem cells for the regeneration of osteoperiosteal critical-size mandibular bony defects, an in vitro and preclinical study Pubmed:24485270
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FEBS Letters Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells Pubmed:25542352
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Experimental And Therapeutic Medicine Antiosteoporotic effect of icariin in ovariectomized rats is mediated via the Wnt/β-catenin pathway Pubmed:27347050
International Journal of Life Sciences Research Impact of Curcumin and Flaxseed Oil on Bone Turnover in Glucocorticoid-Injected Female Rats profile:303311214
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Annals of Animal Science Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens view:87
J Bone Miner Metab MiR-142-5p promotes bone repair by maintaining osteoblast activity Pubmed:27085967
Protective Effect of Soybean and Mushroom against Glucocorticoid-Induced Osteoporosis in Female Rats :
Journal of Functional Foods Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight women S1756464617301561
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Chemico-Biological Interactions Ginsenosides Rg3 attenuates glucocorticoid-induced osteoporosis through regulating BMP-2/BMPR1A/Runx2 signaling pathway pubmed:27387537
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Experimental and Therapeutic Medicine Serum N-terminal telopeptide of type I collagen as an early marker of fracture nonunion in rabbits. pubmed:28105092
Institute of Pharmacology Effects of long-term administration of pantoprazole on bone mineral density in young male rats. pubmed:27552060
Indian Journal of Orthopaedics N-terminal telopeptides of type I collagen and bone mineral density for early diagnosis ofnonunion: An experimental study in rabbits. pubmed:27512225
Biological Trace Element Research Micromolar Levels of Sodium Fluoride Promote Osteoblast Differentiation Through Runx2 Signaling pubmed:28083749
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Biomedicine & Pharmacotherapy Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats S0753332216322752
Biological Trace Element Research Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Diet article:10.1007
Revista Brasileira de Ciência Avícola Effect of Zinc Level and Source (Zinc Oxide Vs. Zinc Glycine) on Bone Mechanical and Geometric Parameters, and Histomorphology in Male Ross 308 Broiler Chicken 10.159:1806-9061-2016-0285
Pharmacological Reports Oral administration of kaempferol inhibits bone loss in rat model of ovariectomy-induced osteopenia pubmed:29031689
Journal of Bone and Mineral Metabolism MiR‑142‑5p promotes bone repair by maintaining osteoblast activity pubmed:27085967
Oncotarget A novel osteoporosis model with ascorbic acid deficiency in Akr1A1 gene knockout mice. pubmed:28060768
Biomedicine & pharmacotherapy Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. pubmed:28742995
Asian Journal of Biomedical and Pharmaceutical Sciences A Water Extract of Benefiting-Bone Capsule Improves Osteoporosis via Transcriptional and Translational Regulation of Key Factors in Notch and Wnt/β-catenin Signaling Pathways in Ovariectomized Rats ISSN 2249 – 622X
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Journal of Cellular Physiology Osteogenic commitment and differentiation of human mesenchymal stem cells by low-intensity pulsed ultrasound stimulation. pubmed:28621452
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