ELISA Kit for Troponin I Type 3, Cardiac (TNNI3)

cTnI; TNNC1; CMH7; C-TnI; Troponin I Type 3; Cardiac troponin I

Specificity

This assay has high sensitivity and excellent specificity for detection of Troponin I Type 3, Cardiac (TNNI3).
No significant cross-reactivity or interference between Troponin I Type 3, Cardiac (TNNI3) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Troponin I Type 3, Cardiac (TNNI3) and the recovery rates were calculated by comparing the measured value to the expected amount of Troponin I Type 3, Cardiac (TNNI3) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-105 96
EDTA plasma(n=5) 96-103 99
heparin plasma(n=5) 93-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Troponin I Type 3, Cardiac (TNNI3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Troponin I Type 3, Cardiac (TNNI3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Troponin I Type 3, Cardiac (TNNI3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 99-105% 96-104% 95-103% 91-105%
EDTA plasma(n=5) 91-101% 80-101% 98-105% 95-103%
heparin plasma(n=5) 93-101% 80-93% 83-93% 79-94%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Animal and Feed Sciences, The effects of caloric restriction and age on thyroid hormone signalling in the heart of rats Pl:Source
Interact Cardiovasc Thorac Surg. Comparison of femoral and aortic remote ischaemia preconditioning for cardioprotection against myocardial ischaemia/reperfusion injury in a rat model Pubmed:25205781
Exp Ther Med Cardioprotective effect of remote preconditioning of trauma and remote ischemia preconditioning in a rat model of myocardial ischemia/reperfusion injury PubMed: 26136887
Heart International Circulating miR-126 and miR-499 reflect progression of cardiovascular disease; correlations with uric acid and ejection fraction pubmed:27924211
Archivos de medicina veterinaria Cardiac biomarkers in dogs with visceral leishmaniasis S0301-732X2016000300004
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA478Ra01 Recombinant Troponin I Type 3, Cardiac (TNNI3) Positive Control; Immunogen; SDS-PAGE; WB.
PAA478Ra01 Polyclonal Antibody to Troponin I Type 3, Cardiac (TNNI3) WB; IHC; ICC; IP.
MAA478Ra21 Monoclonal Antibody to Troponin I Type 3, Cardiac (TNNI3) WB; IHC; ICC; IP.
HEA478Ra High Sensitive ELISA Kit for Troponin I Type 3, Cardiac (TNNI3) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA478Ra ELISA Kit for Troponin I Type 3, Cardiac (TNNI3) Enzyme-linked immunosorbent assay for Antigen Detection.
KSA478Ra01 ELISA Kit DIY Materials for Troponin I Type 3, Cardiac (TNNI3) Main materials for "Do It (ELISA Kit) Yourself".