ELISA Kit for Intercellular Adhesion Molecule 1 (ICAM1)
CD54; BB2; P3.58; Human Rhinovirus Receptor; Major group rhinovirus receptor
- Product No.SEA548Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range78-5,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 30pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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For negotiated price and more details, please contact local distributors! US$ 468
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For negotiated price and more details, please contact local distributors! US$ 3978
For negotiated price and more details, please contact local distributors! US$ 32760
For negotiated price and more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Intercellular Adhesion Molecule 1 (ICAM1).
No significant cross-reactivity or interference between Intercellular Adhesion Molecule 1 (ICAM1) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Intercellular Adhesion Molecule 1 (ICAM1) and the recovery rates were calculated by comparing the measured value to the expected amount of Intercellular Adhesion Molecule 1 (ICAM1) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Intercellular Adhesion Molecule 1 (ICAM1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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|Alexandria Journal of Medicine||Effect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers: An experimental study in Sprague Dawley rats Sciencedirect:S2090506814000268|
|Canadian Journal of Physiology and Pharmacology||Effect of magnesium sulfate and thyroxine on inflammatory markers in a rat model of hypothyroidism Doi: Abs|
|J Cell Biochem||Tumor Necrosis Factor-Alpha Up-Regulates ICAM-1 Expression and Release in Intestinal Myofibroblasts by Redox-Dependent and -Independent Mechanisms PubMed: 26177712|
|Neurotoxicology||Allopurinol reduces severity of delayed neurologic sequelae in experimental carbon monoxide toxicity in rats PubMed: 25845300|
|Alexandria Journal of Medicine||Effect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers: An experimental study in Science: Article|
|Chemical Research in Toxicology||Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation PubMed: 25580880|
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|Journal of Cellular Biochemistry||Tumor Necrosis Factor‐Alpha Up‐Regulates ICAM‐1 Expression and Release in Intestinal Myofibroblasts by Redox‐Dependent and‐Independent Mechanisms pubmed:26177712|
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