ELISA Kit for Intercellular Adhesion Molecule 1 (ICAM1)
CD54; BB2; P3.58; Human Rhinovirus Receptor; Major group rhinovirus receptor
- Product No.SEA548Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.312-20ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.119ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 498
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For more details, please contact local distributors! US$ 34860
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Intercellular Adhesion Molecule 1 (ICAM1).
No significant cross-reactivity or interference between Intercellular Adhesion Molecule 1 (ICAM1) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Intercellular Adhesion Molecule 1 (ICAM1) and the recovery rates were calculated by comparing the measured value to the expected amount of Intercellular Adhesion Molecule 1 (ICAM1) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Intercellular Adhesion Molecule 1 (ICAM1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Amran et al. Lipids in Health and Disease||Changes in the vascular cell adhesion molecule-1, intercellular adhesion molecule-1 and c-reactive protein following administration of aqueous extract of piper sarmentosum on experimental. BioMed: 1476511X|
|Evidence-based Complementary and Alternative Medicine||Preventive Effects of a Natural Anti-Inflammatory Agent, Astragaloside IV, on Ischemic Acute Kidney Injury in Rats PubMed: PMC3703719|
|British Journal of Medical and Health Sciences||EFFECTS OF ADRIAMYCIN, CISPLATIN, AND 5-FLUOROURACIL ON THE TESTES OF ALBINO RATS Researchgate: Source|
|Cytokine||Astragaloside IV ameliorates renal injury in streptozotocin-induced diabetic rats through inhibiting NF-κB-mediated inflammatory genes expression. Pubmed: 23434274|
|BioMed research international||Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations Pubmed: 24199192|
|Journal of Veterinary Internal Medicine||Markers of coagulation activation, endothelial stimulation, and inflammation in dogs with babesiosis Pubmed: 23875771|
|Arch Dis Child||PO-0699 Estimation Of Adhesion Molecules Intrauterine Growth Retardation (iugr) Newborns Bmj:Source|
|Arch Dis Child?||PO-0701 Causes Of Fetal Growth Restriction And Early Neonatal Outcome Bmj:Source|
|Arch Dis Child||PO-0698 Neonatal Resuscitation Practices In The Delivery Room In India: An Audit Using Videography Bmj:Source|
|J Thorac Dis.?||Anti-endothelial cell antibodies in connective tissue diseases associated with pulmonary arterial hypertension Pubmed:Pmc4015009|
|Journal of Immunotoxicology||Acacia ferruginea inhibits cyclophosphamide-induced immunosuppression and urotoxicity by modulating cytokines in mice Pubmed:24873678|
|Free Radical Biology and Medicine||Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis Pubmed:24992835|
|Int J Neurosci.||The effect of therapeutic hypothermia after cardiopulmonary resuscitation on ICAM-1 and NSE levels in sudden cardiac arrest rabbits Pubmed:25111247|
|Naunyn-Schmiedeberg's Archives of Pharmacology||Insights into the antiatherogenic molecular mechanisms of andrographolide against?Porphyromonas gingivalis-induced atherosclerosis in rabbits Pubmed:25172523|
|Nutr Res.||Curcumin attenuates adhesion molecules and matrix metalloproteinase expression in hypercholesterolemic rabbits Pubmed:25282128|
|Experimental & Molecular Medicine||Elevated levels of serum sICAM-1 in asphyxiated low birth weight newborns Pubmed:25358349|
|Neural Regen Res.?||Inflammatory response and neuronal necrosis in rats with cerebral ischemia Pubmed:25422636|
|Chemical Research in Toxicology||Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation Pubmed:25580880|
|CNS Neuroscience & Therapeutics||Peroxisome Proliferator-activated Receptor-γ Agonist Pioglitazone Ameliorates White Matter Lesion and Cognitive Impairment in Hypertensive Rats Pubmed:25611692|
|Alexandria Journal of Medicine||Effect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers: An experimental study in Sprague Dawley rats Sciencedirect:S2090506814000268|
|Canadian Journal of Physiology and Pharmacology||Effect of magnesium sulfate and thyroxine on inflammatory markers in a rat model of hypothyroidism Doi: Abs|
|J Cell Biochem||Tumor Necrosis Factor-Alpha Up-Regulates ICAM-1 Expression and Release in Intestinal Myofibroblasts by Redox-Dependent and -Independent Mechanisms PubMed: 26177712|
|Neurotoxicology||Allopurinol reduces severity of delayed neurologic sequelae in experimental carbon monoxide toxicity in rats PubMed: 25845300|
|Alexandria Journal of Medicine||Effect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers: An experimental study in Science: Article|
|Chemical Research in Toxicology||Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation PubMed: 25580880|
|Veterinary Parasitology||Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis PubMed: 26412140|
|Experimental Eye Research||The effect of triamcinolone acetonide or bevacizumab on the levels of proinflammatory cytokines after retinal laser photocoaCavia (Guinea pig )lation in pigmented rabbits Pubmed:27296072|
|Chinese Chemical Letters||Screening the anti-gout traditional herbs from TCM using an in vitro method science:S1001841716301450|
|Am J Physiol Lung Cell Mol Physiol||Systematic phenotyping and correlation of biomarkers with lung function and histology in lung fibrosis Pubmed:26993522|
|Mol Neurobiol||A Therapeutic Insight of Niacin and Coenzyme Q10 Against Diabetic Encephalopathy in Rats Pubmed:26867655|
|Comparative Clinical Pathology||Identification of some plasma biomarkers associated with early weaning stress in crossbred piglets article:10.1007|
|Journal of Cellular Biochemistry||Tumor Necrosis Factor‐Alpha Up‐Regulates ICAM‐1 Expression and Release in Intestinal Myofibroblasts by Redox‐Dependent and‐Independent Mechanisms pubmed:26177712|
|BMC Vet Res.||Blood markers of fibrinolysis and endothelial activation in canine babesiosis. pubmed:28363279|
|Biomedicine & pharmacotherapy= Biomedecine & pharmacotherapie||Protective effects of N-acetylcystein and atorvastatin against renal and hepatic injury in a rat model of intestinal ischemia-reperfusion pubmed:28273631|
|Pakistan journal of pharmaceutical sciences||Changes in blood pressure, vascular reactivity and inflammatory biomarkers following consumption of heated corn oil. pubmed:29084680|
|Experimental Parasitology||Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis pubmed:28821422|
|Molecular Medicine Reports||Age‑related differences in serum MFG‑E8, TGF‑β1 and correlation to the severity of atherosclerosis determined by ultrasound. pubmed:29152651|
|Journal of Microbiology, Immunology and Infection||Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients pubmed:28655573|
|Journal of Chromatography A||Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis S0021967317311858|
|International Journal of Clinical and Experimental Pathology||Protective effect of high mobility group box-1 silence on diabetic retinopathy: an in vivo study ISSN:1936-2625/IJCEP0055881|
|Oncotarget||Global gene expression profile of cerebral ischemia-reperfusion injury in rat MCAO model pubmed:29088811|
|Microbial Pathogenesis||Protective effects of sinomenine against LPS-induced inflammation in piglets. pubmed:28757275|
|Journal of ophthalmology||Panretinal Photocoagulation Using Short-Pulse Laser Induces Less Inflammation and Macular Thickening in Patients with Diabetic Retinopathy pubmed:28761761|
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