ELISA Kit for Selectin, Platelet (SELP)
CD62P; P-Selectin; PSEL; GMP140; LECAM3; GRMP; GMRP; PADGEM; Leukocyte-endothelial cell adhesion molecule 3; Platelet activation dependent granule-external membrane
- Product No.SEA569Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range78-5,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 35pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Selectin, Platelet (SELP).
No significant cross-reactivity or interference between Selectin, Platelet (SELP) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Selectin, Platelet (SELP) and the recovery rates were calculated by comparing the measured value to the expected amount of Selectin, Platelet (SELP) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Selectin, Platelet (SELP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Selectin, Platelet (SELP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Selectin, Platelet (SELP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Journalof Vascular Research||Effect of Hirulog-Like Peptide on Balloon Catheter Injury-Induced Neointimal Formation in Femoral Arteries of Minipigs and Relationship with Inflammatory Mediators Karger: 000257340|
|Basic Research in Cardiology||Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation PubMed: 22899170|
|BioMed research international||Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations Pubmed: 24199192|
|Free Radical Biology and Medicine||Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis Pubmed:24992835|
|Materials Science and Engineering: C||MRI-compatible Nb–60Ta–2Zr alloy used for vascular stents: Haemocompatibility and its correlation with protein adsorption Pubmed:25063132|
|Blood Coagul Fibrinolysis.?||Chemical sympathectomy attenuates inflammation, glycocalyx shedding and coagulation disorders in rats with acute traumatic coagulopathy. Pubmed:25325345|
|Inflammation.?||Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis Pubmed:25448261|
|Endocrine||Effects of glucagon-like peptide-1 on advanced glycation endproduct-induced aortic endothelial dysfunction in streptozotocin-induced diabetic rats: possible roles of Rho kinase- and AMP kinase-mediated nuclear factor κB signaling pathways Pubmed:26758998|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA569Mu02||Recombinant Selectin, Platelet (SELP)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA569Mu01||Recombinant Selectin, Platelet (SELP)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA569Mu02||Polyclonal Antibody to Selectin, Platelet (SELP)||WB; IHC; ICC; IP.|
|PAA569Mu01||Polyclonal Antibody to Selectin, Platelet (SELP)||WB; IHC; ICC; IP.|
|LAA569Mu71||Biotin-Linked Polyclonal Antibody to Selectin, Platelet (SELP)||WB; IHC; ICC.|
|LAA569Mu81||FITC-Linked Polyclonal Antibody to Selectin, Platelet (SELP)||WB; IHC; ICC; IF.|
|SEA569Mu||ELISA Kit for Selectin, Platelet (SELP)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA569Mu||CLIA Kit for Selectin, Platelet (SELP)||Chemiluminescent immunoassay for Antigen Detection.|