ELISA Kit for P-Selectin (SELP)

CD62P; Selectin, Platelet; PSEL; GMP140; LECAM3; GRMP; GMRP; PADGEM; Leukocyte-endothelial cell adhesion molecule 3; Platelet activation dependent granule-external membrane

Specificity

This assay has high sensitivity and excellent specificity for detection of P-Selectin (SELP).
No significant cross-reactivity or interference between P-Selectin (SELP) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant P-Selectin (SELP) and the recovery rates were calculated by comparing the measured value to the expected amount of P-Selectin (SELP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-104 99
EDTA plasma(n=5) 84-95 89

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level P-Selectin (SELP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level P-Selectin (SELP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of P-Selectin (SELP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-102% 94-102% 85-97% 87-94%
EDTA plasma(n=5) 81-101% 88-96% 82-98% 95-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journalof Vascular Research Effect of Hirulog-Like Peptide on Balloon Catheter Injury-Induced Neointimal Formation in Femoral Arteries of Minipigs and Relationship with Inflammatory Mediators Karger: 000257340
Basic Research in Cardiology Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation PubMed: 22899170
BioMed research international Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations Pubmed: 24199192
Free Radical Biology and Medicine Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis Pubmed:24992835
Materials Science and Engineering: C MRI-compatible Nb–60Ta–2Zr alloy used for vascular stents: Haemocompatibility and its correlation with protein adsorption Pubmed:25063132
Blood Coagul Fibrinolysis.? Chemical sympathectomy attenuates inflammation, glycocalyx shedding and coagulation disorders in rats with acute traumatic coagulopathy. Pubmed:25325345
Inflammation.? Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis Pubmed:25448261
Endocrine Effects of glucagon-like peptide-1 on advanced glycation endproduct-induced aortic endothelial dysfunction in streptozotocin-induced diabetic rats: possible roles of Rho kinase- and AMP kinase-mediated nuclear factor κB signaling pathways Pubmed:26758998
Microbial Pathogenesis Protective effects of sinomenine against LPS-induced inflammation in piglets. pubmed:28757275
Nutrition & Metabolism L-Carnitine Supplementation Increases Trimethylamine-N-Oxide but not Markers of Atherosclerosis in Healthy Aged Women Pubmed: 30485835
Available at SSRN 3335365  Evaluating the Platelet Activation Related to the Degradation of Biomaterials by Scheme of Molecular Markers
Ecotoxicology and Environmental Safety Molecular mechanisms of lead-induced changes of selenium status in mice livers through interacting with selenoprotein P
Thrombosis Research Feasibility study of use of rabbit blood to evaluate platelet activation by medical devices Pubmed: 31838449
JOURNAL OF CONTROLLED RELEASE Polycaprolactone vascular graft with epigallocatechin gallate embedded sandwiched layer-by-layer functionalization for enhanced antithrombogenicity and anti … Pubmed: 31982435
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY The Inhibition of P-Selectin Reduced Severe Acute Lung Injury in Immunocompromised Mice Pubmed: 32377309
blood advances The binding of autotaxin to integrins mediates hyperhomocysteinemia-potentiated platelet activation and thrombosis 34559203
Vaccine The effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female rats Pubmed:35183388
Int J Biol Macromol The inhibition of TRIM35-mediated TIGAR ubiquitination enhances mitochondrial fusion and alleviates renal ischemia-reperfusion injury Pubmed:35421414
Experimental and Therapeutic Medicine Construction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosis Pubmed:35126715
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA569Ra01 Recombinant P-Selectin (SELP) Positive Control; Immunogen; SDS-PAGE; WB.
PAA569Ra01 Polyclonal Antibody to P-Selectin (SELP) WB; IHC; ICC; IP.
LAA569Ra71 Biotin-Linked Polyclonal Antibody to P-Selectin (SELP) WB; IHC; ICC.
MAA569Ra21 Monoclonal Antibody to P-Selectin (SELP) WB; IHC; ICC; IP.
SEA569Ra ELISA Kit for P-Selectin (SELP) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA569Ra Multiplex Assay Kit for P-Selectin (SELP) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.