ELISA Kit for Cyclooxygenase-2 (COX 2)
PGG/HS; PGHS2; PHS2; HCox2; PTGS2; Prostaglandin Endoperoxide Synthase 2; Prostaglandin G/H Synthase And Cyclooxygenase; Prostaglandin H2 synthase 2
- Product No.SEA699Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range78-5,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 35pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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For more details, please contact local distributors! US$ 45360
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This assay has high sensitivity and excellent specificity for detection of Cyclooxygenase-2 (COX 2).
No significant cross-reactivity or interference between Cyclooxygenase-2 (COX 2) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Cyclooxygenase-2 (COX 2) and the recovery rates were calculated by comparing the measured value to the expected amount of Cyclooxygenase-2 (COX 2) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclooxygenase-2 (COX 2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclooxygenase-2 (COX 2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cyclooxygenase-2 (COX 2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Photodermatology, Photoimmunology & Photomedicine||Diallyl sulfide protects against ultraviolet B-induced skin cancers in SKH-1 hairless mouse: analysis of early molecular events in carcinogenesis Wiley: source|
|Skin Cancer - MedlinePlus Health Information||Molecular mechanisms underlying chemopreventive activities of glycyrrhizic acid against UVB-radiation-induced carcinogenesis in SKH-1 hairless mouse epidermis PubMed: 21545294|
|Evidence-Based Complementary and Alternative Medicine||Curcumin Protects against UVB-Induced Skin Cancers in SKH-1 Hairless Mouse: Analysis of Early Molecular Markers in Carcinogenesis PubMed: 22888366|
|Integrative Cancer Therapies||Decalepis hamiltonii inhibits tumor progression and metastasis by regulating the inflammatory mediators and nuclear factor κB subunits Pubmed: 24013642|
|Journal of ethnopharmacology||Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats. Pubmed: 24211397|
|Int J Clin Exp Pathol||Relationship between epidermal growth factor receptor (EGFR) mutation and serum cyclooxygenase-2 Level, and the synergistic effect of celecoxib and gefitinib on EGFR expression in non-small cell lung cancer cells PubMed: 26464643|
|Int J Clin Exp Pathol||Monitoring of cyclooxygenase-2 levels can predict EGFR mutations and the efficacy of EGFR-TKI in patients with lung adenocarcinoma PubMed: 26191267|
|Journal of Biomechanics||The shear wave elastic modulus and the increased nuclear factor kappa B(NF-kB/p65) and cyclooxygenase-2 (COX-2) expression in the area of myofascial trigger points activated in a rat model by blunt trauma to the vastus medialis pubmed:29137729|
|Journal of Global Pharma Technology||Inflammatory Response of Annona Muricata Linn Leaves Extract in Colorectal Cancer Patients NCT02439580|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA699Mu02||Recombinant Cyclooxygenase-2 (COX 2)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA699Mu01||Recombinant Cyclooxygenase-2 (COX 2)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA699Mu01||Polyclonal Antibody to Cyclooxygenase-2 (COX 2)||WB; IHC; ICC; IP.|
|PAA699Mu02||Polyclonal Antibody to Cyclooxygenase-2 (COX 2)||WB; IHC; ICC; IP.|
|LAA699Mu81||FITC-Linked Polyclonal Antibody to Cyclooxygenase-2 (COX 2)||WB; IHC; ICC; IF.|
|LAA699Mu71||Biotin-Linked Polyclonal Antibody to Cyclooxygenase-2 (COX 2)||WB; IHC; ICC.|
|SEA699Mu||ELISA Kit for Cyclooxygenase-2 (COX 2)||Enzyme-linked immunosorbent assay for Antigen Detection.|