ELISA Kit for Prolactin (PRL)

LTH; Luteotropic Hormone

Specificity

This assay has high sensitivity and excellent specificity for detection of Prolactin (PRL).
No significant cross-reactivity or interference between Prolactin (PRL) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Prolactin (PRL) and the recovery rates were calculated by comparing the measured value to the expected amount of Prolactin (PRL) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 98-105 101
EDTA plasma(n=5) 89-98 93
heparin plasma(n=5) 86-103 99

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prolactin (PRL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prolactin (PRL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Prolactin (PRL) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-93% 91-99% 83-101% 96-104%
EDTA plasma(n=5) 84-95% 80-90% 97-105% 88-105%
heparin plasma(n=5) 90-97% 89-98% 98-105% 97-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
The Journal of Biological Chemistry In Vivo Evidence for Epidermal Growth Factor Receptor (EGFR)-mediated Release of Prolactin from the Pituitary Gland Jcb: 39297
Journal of Clinical Neuroscience TLR9 expression is associated with prognosis in patients with glioblastoma multiforme ScienceDirect: S0967586811003572
Sensors and Actuators B: Chemical Determination of prolactin hormone in serum and urine using an electrochemical immunosensor based on poly(pyrrolepropionic acid)/carbon nanotubes hybrid modified electrodes Sciencedirect:S0925400514000720
Veterinary Word Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers Pubmed:27051212
Journal of Dairy Science Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves publication:306322503
Stem Cell Research & Therapy Differentiation of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells into endometrial cells pubmed:29096715
Stem Cell Research & Therapy The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity Pubmed:30021657
Frontiers in Molecular Neuroscience Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice Pubmed:29403355
Toxicology and Applied Pharmacology The antipsychotics sulpiride induces fatty liver in rats via phosphorylation of insulin receptor substrate-1 at Serine 307-mediated adipose tissue insulin resistance Pubmed:29551354
Journal of Cellular Biochemistry The prolactin‐release inhibitor paeoniflorin suppresses proliferation and induces apoptosis in prolactinoma cells via the mitochondria‐dependent pathway Pubmed:29388711
Journal of Cellular and Molecular Medicine Metformin inhibits growth and prolactin secretion of pituitary prolactinoma cells and xenografts Pubmed: 30334324
BMC Veterinary Research Hormonal and metabolic indicators before and after farrowing in sows affected with postpartum dysgalactia syndrome Pubmed: 30404636
International Journal of Basic and Clinical Endocrinology Expression of prolactin receptors in the duodenum, kidneys and skeletal system during physiological and sulpiride-induced hyperprolactinaemia Pubmed: 30143940
MORPHOLOGY AND PATHOMORPHOLOGY Morphological and Biochemical Characteristics of Prostate Hyperplasia during Sulpiride Treatment Pubmed: 32152847
JOURNAL OF DAIRY SCIENCE Effect of heat stress during the early and late dry period on mammary gland development of Holstein dairy cattle Pubmed: 32684470
BioMed Research International Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress Pubmed: 32685488
Vet Microbiol Prolactin affects the disappearance of ALV-J viremia in vivo and inhibits viral infection 34391195
Antioxidants (Basel) Effects of a Nanoencapsulated Moringa Leaf Ethanolic Extract on the Physiology, Metabolism and Reproductive Performance of Rabbit Does during Summer 34439574
Nutrients Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice Pubmed:35057459
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