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ELISA Kit for Resistin (RETN)

ADSF; FIZZ3; RETN1; RSTN; XCP1; Adipose tissue-specific secretory factor; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted FIZZ3

Specificity

This assay has high sensitivity and excellent specificity for detection of Resistin (RETN).
No significant cross-reactivity or interference between Resistin (RETN) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Resistin (RETN) and the recovery rates were calculated by comparing the measured value to the expected amount of Resistin (RETN) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-101 98
EDTA plasma(n=5) 96-104 101
heparin plasma(n=5) 79-102 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Resistin (RETN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Resistin (RETN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Resistin (RETN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 95-102% 90-104% 78-98% 78-99%
EDTA plasma(n=5) 93-101% 79-101% 83-97% 91-99%
heparin plasma(n=5) 83-93% 94-101% 79-99% 87-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
European Journal of Pharmacology Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet PubMed: 20869961
Evid Based Complement Alternat Med. Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar Rats Pubmed:Pmc4363645
J Endocrinol. Estradiol stimulates mitochondrial biogenesis and adiponectin expression in skeletal muscle Pubmed:24681828
Life Sciences Adipose tissue regulates hepatic cholesterol metabolism via adiponectin Pubmed:25445438
Obesity Surgery Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats Pubmed:25697126
Evid Based Complement Alternat Med Tinospora crispa PubMed: 25821506
Journal of Longitudinal changes in adipose tissue of dairy cows from late pregnancy to lactation. Part 1: The adipokines apelin and resistin and their relationship to receptors linked with lipolysis PubMed: 26686707
Genome Genetic Variations in Visfatin and Resistin Genes in Egyptian Type 3 Diabetic Patients With and Without Complications PubMed: 25120108
Acta Histochemica Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines science:S0065128116301234
Journal of Dairy Science Endocrine activity of adipose tissues as influenced by energy intake in the periparturient cow 108495429X:34
Evidence-Based Complementary and Alternative Medicine Four-Week Consumption of Malaysian Honey Reduces Excess Weight Gainand Improves Obesity-Related Parameters in High Fat Diet Induced ObeseRats. pubmed:28246535
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
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