ELISA Kit for Resistin (RETN)
ADSF; FIZZ3; RETN1; RSTN; XCP1; Adipose tissue-specific secretory factor; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted FIZZ3
- Product No.SEA847Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.312-20ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.129ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 498
For more details, please contact local distributors! US$ 2241
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For more details, please contact local distributors! US$ 34860
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Resistin (RETN).
No significant cross-reactivity or interference between Resistin (RETN) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Resistin (RETN) and the recovery rates were calculated by comparing the measured value to the expected amount of Resistin (RETN) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Resistin (RETN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Resistin (RETN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Resistin (RETN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|European Journal of Pharmacology||Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet PubMed: 20869961|
|Evid Based Complement Alternat Med.||Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar Rats Pubmed:Pmc4363645|
|J Endocrinol.||Estradiol stimulates mitochondrial biogenesis and adiponectin expression in skeletal muscle Pubmed:24681828|
|Life Sciences||Adipose tissue regulates hepatic cholesterol metabolism via adiponectin Pubmed:25445438|
|Obesity Surgery||Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats Pubmed:25697126|
|Evid Based Complement Alternat Med||Tinospora crispa PubMed: 25821506|
|Journal of||Longitudinal changes in adipose tissue of dairy cows from late pregnancy to lactation. Part 1: The adipokines apelin and resistin and their relationship to receptors linked with lipolysis PubMed: 26686707|
|Genome||Genetic Variations in Visfatin and Resistin Genes in Egyptian Type 3 Diabetic Patients With and Without Complications PubMed: 25120108|
|Acta Histochemica||Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines science:S0065128116301234|
|Journal of Dairy Science||Endocrine activity of adipose tissues as influenced by energy intake in the periparturient cow 108495429X:34|
|Evidence-Based Complementary and Alternative Medicine||Four-Week Consumption of Malaysian Honey Reduces Excess Weight Gainand Improves Obesity-Related Parameters in High Fat Diet Induced ObeseRats. pubmed:28246535|
|Oncotarget||FAM3A enhances adipogenesis of 3T3-L1 preadipocytes via activation of ATP-P2 receptor-Akt signaling pathway pubmed:28515350|
|Journal of Dairy Science||Involvement of plasma adipokines in metabolic and reproductive parameters in Holstein dairy cows fed with diets with differing energy levels pubmed:28803009|
|Toxicology||Biological effects of adipocytes in sulfur mustard induced toxicity pubmed:29129815|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA847Mu01||Recombinant Resistin (RETN)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA847Mu02||Recombinant Resistin (RETN)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA847Mu01||Polyclonal Antibody to Resistin (RETN)||WB; IHC; ICC; IP.|
|PAA847Mu02||Polyclonal Antibody to Resistin (RETN)||WB; IHC; ICC; IP.|
|LAA847Mu81||FITC-Linked Polyclonal Antibody to Resistin (RETN)||WB; IHC; ICC; IF.|
|LAA847Mu71||Biotin-Linked Polyclonal Antibody to Resistin (RETN)||WB; IHC; ICC.|
|MAA847Mu21||Monoclonal Antibody to Resistin (RETN)||WB; IHC; ICC; IP.|
|SEA847Mu||ELISA Kit for Resistin (RETN)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA847Mu||CLIA Kit for Resistin (RETN)||Chemiluminescent immunoassay for Antigen Detection.|
|KSA847Mu01||ELISA Kit DIY Materials for Resistin (RETN)||Main materials for "Do It (ELISA Kit) Yourself".|