ELISA Kit for Estrogen Receptor Alpha (ERa)

ER-A1; ER; ESR; ESR1; ESRA; NR3A1; NR3-A1; Estrogen Receptor 1; Nuclear Receptor Subfamily 3,Group A,Member 1

Specificity

This assay has high sensitivity and excellent specificity for detection of Estrogen Receptor Alpha (ERa).
No significant cross-reactivity or interference between Estrogen Receptor Alpha (ERa) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Estrogen Receptor Alpha (ERa) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Estrogen Receptor Alpha (ERa) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
The Journal of Steroid Biochemistry and Molecular Biology Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR30 signalling Pubmed:24846829
Biochem Biophys Res Commun Differential expression of estrogen receptor α and β isoforms in multiple and solitary leiomyomas PubMed: 26529545
Journal of Environmental Sciences Bisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblasts science:S1001074216300924
cellular microbiology Influenza A virus NS1 protein-induced JNK activation and apoptosis are not functionally linked. pubmed:28076660
Journal of Steroid Biochemistry and Molecular Biology Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signaling pubmed:28263910
Cellular Physiology and Biochemistry Antiestrogenic Activity of the Xi-Huang Formula for Breast Cancer by Targeting the Estrogen Receptor α Pubmed:29975948
Carleton University Research Virtual Environment In Vitro Study of Mechanisms Underlying the Developmental Effects of Bisphenol A Using Human Fetal Lung Fibroblasts
Clinical Breast Cancer Vitamin D receptor in breast cancer tissues and its relation to estrogen receptor alpha (ER-a) gene expression and serum 25-hydroxyvitamin D levels in Egyptian  Doi: 10.1016/j.clbc.2018.12.019
Journal of geriatric psychiatry and neurology ERα Gene Promoter Methylation in Cognitive Function and Quality of Life of Patients With Alzheimer Disease Pubmed: 30947592
mBio The Effector Domain of the Influenza A Virus Nonstructural Protein NS1 Triggers Host Shutoff by Mediating Inhibition and Global Deregulation of Host Transcription … 34488451
Cardiometry Modifying effect of obesity on the content of sex hormones and their receptors in endometrial adenocarcinoma and its surrounding tissue
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