ELISA Kit for Heat Shock 70kDa Protein 1A (HSPA1A)

HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70

Specificity

This assay has high sensitivity and excellent specificity for detection of Heat Shock 70kDa Protein 1A (HSPA1A).
No significant cross-reactivity or interference between Heat Shock 70kDa Protein 1A (HSPA1A) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Heat Shock 70kDa Protein 1A (HSPA1A) and the recovery rates were calculated by comparing the measured value to the expected amount of Heat Shock 70kDa Protein 1A (HSPA1A) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 91-104 101
EDTA plasma(n=5) 83-90 86
heparin plasma(n=5) 84-102 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock 70kDa Protein 1A (HSPA1A) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock 70kDa Protein 1A (HSPA1A) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Heat Shock 70kDa Protein 1A (HSPA1A) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-89% 91-101% 85-99% 89-101%
EDTA plasma(n=5) 96-105% 89-103% 89-104% 91-102%
heparin plasma(n=5) 82-103% 78-96% 92-103% 89-98%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Neuroinflammation Identification of potential biomarkers of gold nanoparticle toxicity in rat brains PubMed: 22691312
Mediators Inflamm.? Local Inflammation in Fracture Hematoma: Results from a Combined Trauma Model in Pigs Pubmed:Pmc4324980
Eur J Pharmacol. Differential modulatory effects of morphine on acute and chronic stress induced neurobehavioral and cellular markers in rats Pubmed:24508522
Pharmacol Rep. Effect of propranolol on IL-10, visfatin, Hsp70, iNOS, TLR2, and survivin in amelioration of tumor progression and survival in Solid Ehrlich Carcinoma-bearing mice Pubmed:25443743
Advances in Medical Sciences A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmatics pubmed:28822267
Archives of Medical Research Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young … Pubmed:29379533
metabolic brain disease Curcumin attenuates cytoplasmic/endoplasmic reticulum stress, apoptosis and cholinergic dysfunction in diabetic rat hippocampus Pubmed: 32172517
EXPERIMENTAL CELL RESEARCH Antagomirs targeting miR-142–5p attenuate pilocarpine-induced status epilepticus in mice Pubmed: 32439493
Catalog No. Related products for research use of Bos taurus; Bovine (Cattle) Organism species Applications (RESEARCH USE ONLY!)
RPB081Bo01 Recombinant Heat Shock 70kDa Protein 1A (HSPA1A) Positive Control; Immunogen; SDS-PAGE; WB.
PAB081Bo01 Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) WB; IHC; ICC; IP.
LAB081Bo81 FITC-Linked Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) WB; IHC; ICC; IF.
LAB081Bo71 Biotin-Linked Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) WB; IHC; ICC.
MAB081Bo21 Monoclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) WB; IHC; ICC; IP.
SEB081Bo ELISA Kit for Heat Shock 70kDa Protein 1A (HSPA1A) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB081Bo Multiplex Assay Kit for Heat Shock 70kDa Protein 1A (HSPA1A) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB081Bo01 ELISA Kit DIY Materials for Heat Shock 70kDa Protein 1A (HSPA1A) Main materials for "Do It (ELISA Kit) Yourself".