ELISA Kit for Superoxide Dismutase 2, Mitochondrial (SOD2)

IPO-B; MNSOD; Mn-SOD

Specificity

This assay has high sensitivity and excellent specificity for detection of Superoxide Dismutase 2, Mitochondrial (SOD2).
No significant cross-reactivity or interference between Superoxide Dismutase 2, Mitochondrial (SOD2) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Superoxide Dismutase 2, Mitochondrial (SOD2) and the recovery rates were calculated by comparing the measured value to the expected amount of Superoxide Dismutase 2, Mitochondrial (SOD2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-98 94
EDTA plasma(n=5) 90-101 95
heparin plasma(n=5) 91-98 95

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Superoxide Dismutase 2, Mitochondrial (SOD2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Superoxide Dismutase 2, Mitochondrial (SOD2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Superoxide Dismutase 2, Mitochondrial (SOD2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 95-105% 86-102% 91-98% 78-102%
EDTA plasma(n=5) 83-97% 85-97% 87-101% 80-102%
heparin plasma(n=5) 85-99% 83-97% 94-103% 89-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Oral Disease Antioxidant profile of salivary glands in high fat diet-induced insulin resistance rats Pubmed: 24106991
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Arch Oral Biol Antioxidant profile, carbonyl and lipid oxidation markers in the parotid and submandibular glands of rats in different periods of streptozotocin induced diabetes PubMed: 26143097
J Oral Pathol Med Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human saliva PubMed: 26608886
Free Radic Biol Med. DDAH1 deficiency promotes intracellular oxidative stress and cell apoptosis via a miR-21-dependent pathway in mouse embryonic fibroblasts. Pubmed:26806551
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Biomolecules Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease Development Pubmed: 32751983
Nutrients Attenuation of Oxidative Stress and Inflammatory Response by Chronic Cannabidiol Administration Is Associated with Improved n-6/n-3 PUFA Ratio in the White and?¡­ 34064937
Pharmaceuticals Serum Total SOD Activity and SOD1/2 Concentrations in Predicting All-Cause Mortality in Lung Cancer Patients 34832849
Biochimica et Biophysica Acta-Molecular Basis of Disease α-Lipoic acid ameliorates inflammation state and oxidative stress by reducing the content of bioactive lipid derivatives in the left ventricle of rats fed a high-fat diet Pubmed:35569738
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