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ELISA Kit for Low Density Lipoprotein (LDL)


This assay has high sensitivity and excellent specificity for detection of Low Density Lipoprotein (LDL).
No significant cross-reactivity or interference between Low Density Lipoprotein (LDL) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Low Density Lipoprotein (LDL) and the recovery rates were calculated by comparing the measured value to the expected amount of Low Density Lipoprotein (LDL) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-98 94
EDTA plasma(n=5) 89-96 92
heparin plasma(n=5) 81-98 84


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Low Density Lipoprotein (LDL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Low Density Lipoprotein (LDL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Low Density Lipoprotein (LDL) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 96-103% 90-101% 80-95% 86-102%
EDTA plasma(n=5) 88-95% 78-92% 88-99% 87-104%
heparin plasma(n=5) 94-102% 85-99% 82-97% 78-99%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
PloS one Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL. NCBI: PMC3896367
PLoS One. Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL Pubmed:24465540
J Lipid Res PCSK9 deficiency unmasks a sex-and tissue-specific subcellular distribution of the LDL and VLDL receptors in mice PubMed: 26323289
Neuroscience 27-Hydroxycholesterol contributes to disruptive effects on learning and memory by modulating cholesterol metabolism in the rat brain PubMed: 25987203
The Journal of Nutritional Biochemistry Protective role of n6/n3 PUFA supplementation with varying DHA/EPA ratios against atherosclerosis in mice Pubmed:27142749
 Journal of Functional Foods Red paprika (Capsicum annuum L.) and its main carotenoid capsanthin ameliorate impaired lipid metabolism in the liver and adipose tissue of high-fat diet-induced obese mice S1756464617300555
Int J Mol Sci. Combined Effects of Curcumin and Lycopene or Bixin in Yoghurt on Inhibition of LDL Oxidation and Increases in HDL and Paraoxonase Levels in Streptozotocin-Diabetic Rats. pubmed:28333071
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