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ELISA Kit for A Disintegrin And Metalloprotease 17 (ADAM17)
CD156b; TACE; CSVP; Tumor Necrosis Factor,Alpha,Converting Enzyme; Disintegrin and metalloproteinase domain-containing protein 17; Snake venom-like protease
- Product No.SEB555Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.057ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 368
US$ 525
US$ 2363
US$ 4463
US$ 36750
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 17 (ADAM17).
No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 17 (ADAM17) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant A Disintegrin And Metalloprotease 17 (ADAM17) and the recovery rates were calculated by comparing the measured value to the expected amount of A Disintegrin And Metalloprotease 17 (ADAM17) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 78-98 | 90 |
| EDTA plasma(n=5) | 78-92 | 85 |
| heparin plasma(n=5) | 81-102 | 86 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 17 (ADAM17) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 17 (ADAM17) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of A Disintegrin And Metalloprotease 17 (ADAM17) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-103% | 93-101% | 95-103% | 94-102% |
| EDTA plasma(n=5) | 82-101% | 96-103% | 84-96% | 79-91% |
| heparin plasma(n=5) | 95-103% | 94-104% | 80-98% | 96-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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| Magazine | Citations |
| Int Wound J. | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. Pubmed:26991748 |
| International Wound Journal | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases pubmed:26991748 |
| Biomedical Research | ADAM17 regulates TNF-α expression upon lipopolysaccharide stimulation in oral keratinocytes. pubmed:28637950 |
| Cytometry B Clin Cytom | L‐Selectin Expression Is Influenced by Phosphatase Activity in Chronic Lymphocytic Leukemia Pubmed: 30729673 |
| Disease Markers | Adamalysines as Biomarkers and a Potential Target of Therapy in Colorectal Cancer Patients: Preliminary Results Pubmed: 31565100 |
| Chem Biol Interact | PEG-SOD attenuates the mitogenic ERK1/2 signaling cascade induced by cyclosporin A in the liver and kidney of albino mice Pubmed: 32866465 |
| life | High Levels of TNF-¦Á and TIM-3 as a Biomarker of Immune Reconstitution Inflammatory Syndrome in People with HIV Infection |