ELISA Kit for Fusion (FUS)

ALS6; TLS; hnRNP-P2; Amyotrophic Lateral Sclerosis 6; Heterogeneous Nuclear Ribonucleoprotein P2; 75 kDa DNA-pairing protein; Translocated in liposarcoma protein; CHOP

Specificity

This assay has high sensitivity and excellent specificity for detection of Fusion (FUS).
No significant cross-reactivity or interference between Fusion (FUS) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fusion (FUS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fusion (FUS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Medicine (Baltimore) Levels of circulating GRP78 and CHOP in endoplasmic reticulum stress pathways in Chinese type 2 diabetic kidney disease patients 34414940
Therapeutic Advances in Endocrinology and Metabolism Circulating microRNA-194 levels in Chinese patients with diabetic kidney disease: a case–control study 34676065
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPC260Mu01 Recombinant Fusion (FUS) Positive Control; Immunogen; SDS-PAGE; WB.
RPC260Mu02 Recombinant Fusion (FUS) Positive Control; Immunogen; SDS-PAGE; WB.
PAC260Mu02 Polyclonal Antibody to Fusion (FUS) WB; IHC; ICC; IP.
PAC260Mu01 Polyclonal Antibody to Fusion (FUS) WB; IHC; ICC; IP.
LAC260Mu81 FITC-Linked Polyclonal Antibody to Fusion (FUS) WB; IHC; ICC; IF.
LAC260Mu71 Biotin-Linked Polyclonal Antibody to Fusion (FUS) WB; IHC; ICC.
SEC260Mu ELISA Kit for Fusion (FUS) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC260Mu Multiplex Assay Kit for Fusion (FUS) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.